【作者】 丁娟娟； 王邦茂； 方维丽； 曹晓沧；

【Author】 Ding Juan-Juan Wang Bang-Mao Fang Wei-Li Cao Xiao-Cang Department of Gastroenterology, Tianjin Medical University Affiliated General Hospital Tianjin 300052, China

【机构】 天津医科大学总医院消化科；

【摘要】 目的:胰岛素样生长因子(insulin-like growth factors,IGFs)在恶性肿瘤的发生、发展过程中发挥重要作用, 主要通过IGF—IR发挥生物学效应。本实验探讨IGF—I及其受体在胃癌组织中的表达情况及临床意义。方法:应用免疫组织化学方法检测40例原发性胃癌癌组织及其自身正常断端胃组织中IGF—I和IGF—IR(IGF-I／IGF—IR)的表达,并分析其表达与胃癌病理分级、临床分期、淋巴结转移、肿瘤侵袭深度的关系。此外,应用蛋白免疫印迹法(western blot)检测胃癌细胞系SGC7901,MGC803的IGF-IR的表达。结果: 胃癌组织IGF—I／IGF—IR的阳性率分别为75％,75．5％,均显著高于手术切缘正常胃组织的阳性率27．5％,25％(P<0． 05)。IGF—I／IGF—IR的表达与性别、年龄、分化程度无关, TNM分期(III+IV)期胃癌组织的IGF-I／IGF—IR蛋白表达水平均高于(I+II)期(P<0．05), 伴有淋巴结转移胃癌组织中的表达明显高于无淋巴结转移组织(P<0．05),肿瘤浸润深度越深,IGF-I／IGF—IR的表达越高,但未达到统计学水平(P>0．05)。IGF-I／IGF—IR在胃癌组织中的蛋白表达水平呈正相关。胃癌细胞系SGC7901、MGC803的IGF— 1R均为阳性表达。结论:IGF-I／IGF—IR在胃癌组织中过表达,蛋白表达水平与胃癌TNM分期,转移能力有关,提示 IGF-I／IGF-IR的过表达胃癌的发生发展发挥重要作用。更多还原

【Abstract】 Objective: The insulin growth factors(IGFs) are mitogens that play key roles the development and progression of neoplasia. The effects of IGFs are mediated mainly through the IGF-I receptor. In order to explore the significance of IGF-I and IGF-IR(IGF-I/IGF-IR) in human gastric cancer,the expression of IGF-I/IGF-IR were studied. Methods: we analyzed the expression of IGF-I/IGF-IR in 40 paired samples of normal and carci-nomatous gastric tissue by immunohistochemistry. The relationship of expression of IGF-I/IGF-IR with various clinicopathological parameters were analyzed. The expression of IGF-IR in gastric cancer cells SGC7901and MGC803 were measured by western blot. Results: As measured by immunohistochemistry, the positive rates of IGF-I and IGF-IR expressions in tumor were 75% and 75.5%, significantly higher than 27.5% and 25% in normal tissues, respectively( P<0. 05). No correlation were found between expression level of IGF-I/IGF-IR and patients sex,age and histological differentiation of gastric cancer.The postive rate of IGF-I/IGF-IR protein expression was higher in stage Ⅲ + IV of gastric cancer than in stage Ⅰ + Ⅱ of gastric cancer and was higher in gastric cancer with lymphnode metastasis than in those without lymphnode metastasis.The deeper extent of infiltrating,the higher IGF-I/IGF-IR positive rate was,but didn’ t reach the statistical level.The expression of IGF-IR of gastric cancer cells SGC7901 and MGC803 were postive. A positive correlation was observed between the overexpression of IGF-I and IGF-IR in the tumors . Conclusion: Our results demonstrate that a strong overexpression of IGF-I/IGF-IR is found in the majority of gastric cancer, supporting the hypothesis of an important role of the IGF system in the pathogenesis gastric cancer.更多还原