【作者】 张鹏； 刘毅梅； 陈洁； 宋世辉； 商忠良；

【Author】 Zhang Peng Liu Yi-Mei Chen Jie Song Shi-Hui Shang Zhong-Liang Department of Cardiothoracic Surgery, General Hospital, Tianjin Medical University , Tianjin 300052, China

【机构】 天津医科大学总医院心胸外科；

【摘要】 目的:检测非小细胞肺癌患者LUNX mRNA的表达情况,探讨其作为微转移检测分子标志物的可行性。方法: 选择初治的非小细胞肺癌(NSCLC)患者62例,以肺部良性疾病10例与健康人10例作为对照组,采用实时荧光定量逆转录聚合酶链反应(FQ—RT—PCR)和普通RT-PCR技术,检测肺癌,良性病变肺组织,骨髓和外周血的LUNX mRNA 的表达。结果:肺癌和良性病变肺组织RT—PCR检测结果显示LUNX mRNA表达阳性率为100％,肺良性病变组骨髓及外周血标本和健康对照组外周血标本FQ—RT—PCR检测结果显示LUNX mRNA表达皆为阴性,62例NSCLC患者骨髓及外周血标本FQ—RT—PCR检测结果显示,LUNX mRNA 表达阳性率38．7％(24／62),外周血LUNX mRNA表达阳性率29．0％(18／62),至少一项表达阳性率45．2％(28／62),骨髓 LUNX mRNA表达阳性率随分期升高且有统计学意义(P=0．02), 而与病理类型,肿瘤分化程度无关,外周血LUNX mRNA 表达与病理分期,类型,肿瘤分化程度无关。骨髓和外周血 LUNX mRNA表达明显相关(P<0．001)。结论:LUNX mRNA可作为检测非小细胞肺癌患者微转移特异,敏感的分子标志物,可能有助于早期诊断肺癌转移,从而指导临床分期和治疗。更多还原

【Abstract】 Objective: Detection of LUNX mRNA in non - small cell lung cancer (NSCLC) patients in order to discuss the possibility of indicating lung cancer micrometastases by LUNX. Methods: Fluorescence quantitative reverse transcription-polymerase chain reaction (FQ-RT-PCR) and ordinary RT- PCR was used to detect LUNX mRNA in cancer tissue, bone marrow and peripheral blood from 62 patients with NSCLC. Lung tissue, bone marrow and peripheral blood from 10 patients with benign pulmonary diseases and peripheral blood from 10 healthy volunteers were served as controls. Results: LUNX mRNA was expressed in all the lung tissues, either malignant or benign. No sample was positive for LUNX mRNA in controls. The positive detection rate of LUNX mRNA for NSCLC was 38.7% (24/62) in bone marrow, 29. 0% (18/62) in peripheral blood, and 45.2%(28/62) in either. The positive rate of LUNX mRNA for NSCLC in bone marrow increases according to the stage of disease and there is a statistical significance (P=0.02), and for the sample expression of LUNX mRNA in NSCLC there is a relationship between bone marrow and peripheral blood(P<0.001). Conclusion: LUNX mRNA is an efficient indicating factor on sensitivity and specificity to detect early haematogenous dissemination of cancer cells for patients with NSCLC. This method may lead to an earlier diagnosis of metastasis for lung cancer and do some help to evaluate the cancer more correctly and make the best treatment plan.更多还原