【作者】 苏延军； 任凯； 王长利；

【Author】 SuYan-Jun Ren Kai Wang Chang-Li Department of Lung Cancer, Tianjin Medical University Cancer Institute and Hospital, Tianjin 300060, China

【机构】 天津医科大学附属肿瘤医院肺部肿瘤科；

【摘要】 目的:探讨CD4+CD25+调节性T细胞在肺癌的发生发展中的作用及其与肺癌患者淋巴结免疫功能变化的关系。方法:手术切除53例非小细胞肺癌患者引流区淋巴结,采用双标记的间接免疫荧光法检测淋巴结中CD4+CD25+调节性 T细胞的数量。结果:非小细胞肺癌患者引流区转移淋巴结中调节性T细胞的数量(28．80±8．06％)明显高于未转移淋巴结(15.48±4．66％,P<0．01)。随肺癌的进展,即纵隔淋巴结转移,肿瘤生长越大,肿瘤分期越晚,淋巴结中调节性 T细胞的数量越多。转移的纵隔淋巴结(N2)和转移的肺内淋巴结(N1)中调节性T细胞的数量分别为32．58±7．52％和22．76±4．67％(P<0．01)。肿瘤较大(T2+T3)和肿瘤较小(T1)的患者淋巴结中调节性T细胞的数量分别为29． 67±7．57％和15．75±4．85％(P<0．01)。进展期(III)和早期(I+II)非小细胞肺癌患者淋巴结中调节性T细胞的数量分别为30．42±7．47％和16．22±4．88％(P<0．01)。结论: 肺癌患者引流区淋巴结中,肿瘤浸润淋巴结的CD4+CD25+调节性T细胞数量明显升高,且与肺癌的发展密切相关。肺癌患者引流区淋巴结呈现免疫抑制状态。检测肺癌患者引流区淋巴结的免疫状况为我们开辟了一条新的途径去评价肺癌患者的预后。更多还原

【Abstract】 Objective: The current study was designed to measure the prevalence of CD4+CD25+ T regulatory cells in draining lymph nodes of NSCLC and to find out the role that CD4+CD25+ T regulatory cells played in the regulation of immune status in draining lymph nodes and the progression of NSCLC. Methods: 53 draining lymph nodes of NSCLC were acquired by operation. The percentage of CD4+CD25+ T regulatory cells in CD4+ T lymphocytes from lymph nodes was analyzed by indirect im-munofluorescence method. Results: The population of CD4+CD25+ T regulatory cells in draining lymph nodes of NSCLC was higher in the tumor-infiltrating lymph nodes (28.80 ± 8.06%) than tumor-free lymph nodes(15.48 ?4. 66%, P < 0.01). It was also higher in the progression of NSCLC, such as mediastinal lymph node metastasis, larger tumor size and advanced TNM staging. The population of CD4+CD25+ T regulatory cells was 32.58 ± 7.52% and 22. 76 ± 4.67%, respectively, (P < 0.01) in positive N2 and N1. It was 29.67 ± 7.57% and 15.75 ± 4.85%, respectively, (P < 0.01) in the lymph nodes of the patients with large tumor size(T2+T3) and small tumor size(T1). In advanced stage(III) and early stage(I+II) of NSCLC, it was 30.42 ± 7.47% and 16.22 ± 4.88%, respectively, (P < 0.01). Conclusion: The significant increasing of the population of CD4+CD25+ T regulatory cells in draining lymph nodes of NSCLC showed the immunosuppressive status in tumor-draining lymph nodes, which closely correlated with the progression and infiltration of lung cancer. Consequently, we could establish a new pathway that helped us to evaluate the prognosis of lung cancer with measuring the immune status in draining lymph nodes of lung cancer in the clinical practice.更多还原