【作者】 罗元； 赵惠柳； 莫立根； 邝国乾； 曹骥； 黄光武； 王会河；

【Author】 Luo Yuan Zhao Hui-Liu Mo li-Gen Kuang Guo-Qian Huang Guan-Gwu Wang Hui-He Department of pathology, Guangxi Cancer Hospital, Nanning 530021, China

【机构】 广西壮族自治区肿瘤医院病理科；

【摘要】 目的:探讨Tiam-1mRNA及其蛋白的表达与鼻咽癌浸润转移的关系及意义。方法:41例不同临床分期人鼻咽癌组织以及对照组20例鼻咽慢性炎症黏膜组织标本,用RT- PCR方法检测Tiam-1 mRNA表达水平,并用免疫组化检测Tiam-1蛋白表达情况。结果:Tiam-1 mRNA在鼻咽癌组织的平均表达水平为1．83±0．73,明显高于鼻咽粘膜慢性炎组织0．87±0．45(P<0．01);而且鼻咽癌组Tiam-1 mRNA高表达率为46．34％,明显高于对照组0％(P<0．01)。 Tiam-1蛋白在鼻咽癌组阳性率为65．85％(27／41),明显高于对照组15％(3／20)(P<0．01);其高表达率为43．90％(18／ 41),明显高于对照组0％(P<0．01)。Tiam-1 mRNA及其蛋白高表达率在临床TNM各分期间的差异均有明显统计学意义(P<0．01);在各T分期之间的差异亦有明显统计学意义 (P<0．01)。鼻咽癌中有淋巴结转移组Tiam-1 mRNA及其蛋白高表达率分别为59．26％(16／27)及55．56％(15／27), 均明显高于无淋巴结转移组的21．43％(3／14)及14．28(2／ 14),(P<0．05)。结论:Tiam-1mRNA及其蛋白高表达与鼻咽癌浸润转移有密切关系,提示Tiam-1基因可能是鼻咽癌的一个重要的促浸润转移因子,有望成为治疗的靶标及有价值的预后判断指标。更多还原

【Abstract】 Objective: To investigate the relationship and significance of expression of Tiam-1 mRNA and Tiam-1 protein in invasion and metastasis of nasopharyngeal carcinoma (NPC). Methods: Expression of Tiam-1mRNA and protein in 41 cases of NPC tissue and 20 cases of chronic nasopharyngitis (CN) tissue as the control group were evaluated by semi-quantitative RT-PCR and immunohistochemistry. Results: The expression level of Tiam-1 mRNA was significantly higher in NPC(1.83 ± 0.73)than that in CN(0,87 ± 0.45) (P < 0.01).The overexpression rates of both Tiam-1 mRNA and protein in NPC tissues were higher than that in CN tissues (P < 0.01) and positively correlated with TNM stages (P < 0.01) and T stages (P < 0.01). In group without lymph node metastasis of NPC and group with lymph node metastasis, the frequency of Tiam-1 mRNA and protein overexpression rates were 21.43%(3/14) and 59.26%(16/ 27); 14.28%(2/14) and 55.56%(15/27), which showed very significancy( p < 0.05). Conclusion: The overexpression of both Tiam-1 mRNA and protein significantly relate to the invasion and metastasis of NPC. Tiam-1 gene may play an important role in accelerating invasion and metastasis of NPC, which may become a target of treatment and prognostic biomarker in NPC.更多还原