【作者】 陈文森； 王莉娜； 靳光付； 王建明； 华召来； 谈永飞； 周炎； 丁永良； 沈靖； 徐耀初； 沈洪兵；

【Author】 Chen Wen-Sen Wang Li-Na Jin Guang-Fu Wang Jian-Min Hua ZaoLai Tan Yong-Fei Zhou Yan Ding Yong-Liang Shen Jing Xu Yao-Chu Shen Hong-Bing Department of Epidemiology and Biostatistics, Nanjing Medical University School of Public Health, Nanjing 210029, China Yangzhong Cancer Institute, Yangzhong City 210623, Jiangsu Province, China Yixing People’ s Hospital, Yixing City 214200, Jiangsu Province, China

【机构】 南京医科大学流行病与卫生统计学系； 扬中市肿瘤研究所； 宜兴市人民医院；

【摘要】 目的:探讨胰岛素样生长因子结合蛋白3(Insulin— like growth factor binding-protein3,IGFBP-3)基因多态性与胃癌易感性的关系。方法:以人群为基础的病例对照研究,经组织学确诊的胃癌高发区病例576人,并在同地区选择与病例年龄和性别频数匹配的人群对照647人,用PCR- 限制性片段长度多态性(R F L P)进行基因型检测,比较 IGFBP-3 A-202C、IGFBP-3 G2132C不同基因型与胃癌风险的关系,并探讨吸烟、饮酒等环境因素在其中的影响。结果:携带IGFBP-3 2132GC及CC基因型是GG基因型发生胃癌风险性的1．84和2．39倍(校正OR=1．84,95％CI:1．45- 2．33;2．39,95％CI:1．47-3．90)。显性模型研究显示,携带 IGFBP一3 2132GC／CC基因型发生胃癌的风险是GG基因型的1．90倍(校正OR=1．90,95％CI:1．51-2．39);未观察到 IGFBP-3A-202C基因多态性对胃癌危险性的影响。但是两位点(IGFBP-3 A-202C和IGFBP-3 G2132C)之间存在明显的交互作用(Pint<0．0001)。未发现吸烟、饮酒与IGFBP-3 基因多态性在胃癌发生上的交互作用。结论:IGFBP-3 G2132C多态性与胃癌的遗传易感性之间存在关联,并且在两位点(IGFBP-3 A-202C和IGFBP-3 G2132C)之间存在比较明显的位点与位点间的交互作用。更多还原

【Abstract】 Objective: To determine whether genetic polymorphisms in insulin-like growth factor binding-protein 3 (IGFBP-3A-202C and IGFBP-3G2132C) were associated with the risks of gastric cancer. Methods: A hospital-based case-control study consisting of 576 incident gastric cancer cases and 647 controls matched on age, sex and race was conducted to investigate the association between polymorphisms in IGFBP-3 and susceptibility to gastric cancer. Genotypes of IGFBP-3 A-202C and G2132C were analyzed by polymerase chain reaction-restriction fragment length polymorphism methods. Results: No significant difference was found for genotype frequencies of A-202C polymorphism between GC cases and controls (P=0.423), while for another polymorphism (G2132C), the variant alleie C remarkably increased the risk of GC (adjusted OR=2.39; 95% CI, 1.47-3.90 for 2132CC, adjusted OR=1.84; 95% CI, 1.45-2.33 for 2132GC, and adjusted OR=1.90, 95 %CI, 1.51-2.39 for 2132GC/CC ), compared with 2132GG wild-type homozygote. Meanwhile, a peculiar locus-locus interaction was found between IGFBP-3 A-202C and IGFBP-3 G2132C (Pint <0.0001), which appeared an antagonistic effects. Conclusion: These data strongly suggested that the G2132C variant in exonl of IGFBP-3 may be an important susceptibility marker for gastric cancer and further functional studies are needed to confirm our findings.更多还原