【作者】 王瑞； 曾辉； 李琰； 王娜； 彭伟； 张健慧； 刘俊锋；

【Author】 Wang Rui ZengHui Li Van etal. The Fourth Hospital of Hebei medical university & Hebei provincial tumor hospital, Shijiazhuang, 050011, China

【机构】 河北医科大学第四医院暨河北省肿瘤医院胸外科；

【摘要】 目的:XRCC2参与同源重组修复,其基因多态性与乳腺癌、基底细胞癌等肿瘤易感性有关。本研究探讨中国北方人群中XRCC2 C41657T、G4234C基因多态性与肺癌易感性的关系。方法:应用PCR—RFLP方法检测199例肺癌患者和200例正常人的XRCC2 C41657T及G4234C多态位点,比较两组之间等位基因及基因型频率分布。结果:肺癌患者XRCC2 C41657T多态位点的CC、CT、TT基因型和C、T等位基因频率分布与健康对照组棚比均无显著性差异(P>0．05)。G4234C多态位点的GG、GC、CC基因型和 G、C等位基因频率分布与健康对照组相比也均无显著性差异(P>0．05)。两多态性位点联合分析显示,肺癌患者与健康对照组的4个单体型分布亦无显著性差异(P>0．05)。以病理类型、吸烟状况和年龄进行分层分析显示,XRCC2 C41657T多态位点可能与腺鳞癌和不吸烟人群的肺癌发病风险相关,与C／C基因型相比,携带T等位基因的基因型(C／ T+T／T)可显著增加腺鳞癌的发病风险(OR为2．84, 95％CI=1．11～7．25);而C／T基因型可显著增加不吸烟组人群中肺癌的发病风险(OR为2．12,95％CI=1．05～4．27)。 XRCC2 G4234C多态位点可能与小细胞癌和肺癌的发病年龄相关,与G／G基因型相比,G／C基因型和携带C等位基因的基因型(G／C+C／C)可显著增加小细胞癌的发病风险 (OR为2．69和2．70;95％CI=1．12～6．49和1．15～6．36); G／C基因型或与C／C基因型相加可显著增加年龄≥60岁的人群中肺癌的发病风险(OR为2．29和2．37;95％CI=1．14～ 4．62和1．18～4．75)。结论:对于XRCC2 C41657T多态位点,携带T等位基因的基因型可能增加腺鳞癌的发病风险,C／T基因型可能增加不吸烟者患肺癌风险。就XRCC2 G4234C多态位点而言,G／C基因型或携带C等位基因可能增加小细胞癌的发病风险和老年人(年龄≥60岁)患肺癌的风险。更多还原

【Abstract】 Objective: XRCC2 is involved in the homologous recombination, its polymorphisms have been thought to be related to susceptibility of breast cancer, BCC (basal cell carcinoma), and so on. This study was designed to investigate the possible association of functional polymorphisms of XRCC2 C41657T and G4234C genes with susceptibility of lung cancer in north China. Methods: The single nucleotide polymorphisms (SNP) in XRCC2 C41657T and G4234C were genotyped by PCR-RFLP analysis in 199 patients with lung cancer, and 200 healthy controls. The genotype and allele distribution were compared. Results: The genotype and allele distribution of the XRCC2 C41657T and G4234C SNP in the patients with lung cancer was not significantly different from that in healthy controls (all P>0.05). When the two XRCC2 SNP were combined, the haplotype distribution in lung cancer patients was not significantly different from that in healthy controls (P>0.05). Stratification analysis according to his-tological type, smoking status, and age showed that compared with C/C genotype, the combination of C/T and T/T genotypes of XRCC2 C41657T increased the risk of adenosquamous carcinoma (OR=2.84, 95%CI=1.11 -7.25), and the C/T genotype of XRCC2 C41657T increased the risk of lung cancer in non-smokers (OR=2.12, 95%CI=1.05-4.27). Compared with G/G genotype, the combination of G/C and C/C of XRCC2 G4234C and G/C genotype both increased the risk of small cell lung cancer (OR=2.69,2.70; 95%CI=1.12 - 6.49,1.15 - 636). For old subjects (age ≥ 60 years), the combination of G/C and C/ C of G4234C and G/C genotype both increased the risk of lung cancer. (OR=2.29,2.37,95%CI= 1.14 - 4.62,1.18 -4.75 X Conclusion: In the site of XRCC2 C41657T SNP, the genotype what carried with T allele increased the risk of adenosquamous carcinoma, the C/T genotype increased the risk of lung cancer in non-smokers. In the site of G4234C SNP, the G/C genotype and the genotype what carried with C allele both increased the risk of lung cancer among old subjects (age ≥ 60 years)and small cell lung cancer.更多还原