【作者】 姜达； 刘鹏飞； 董秀娟； 洪雷； 李琰； 王娜； 郭玮；

【Author】 Jiang Da Liu Peng-Fei Dong Xiu-Juan et al the fourth hospital of Hebei medical university & Hebei provincial tumor hospital, Shijiazhuang, 050011, China

【机构】 河北医科大学大学第四医院暨河北省肿瘤医院肿瘤内科；

【摘要】 目的:卵巢癌是常见的妇科恶性肿瘤之一,90％以上来自上皮组织。XRCC3基因是RAD51基因家族中的新成员,参与DNA双链断裂的重组修复,对于维持基因组功能完整性,修复DNA损伤有着重要作用,该基因存在单核苷酸多态性(SNP)位点,可能影响其修复能力及与肿瘤的发病风险相关。本研究旨在探讨XRCC3 A17893G SNP与卵巢癌易感性的关系。该基因多态性的研究可望成为卵巢癌早期诊断的重要线索。方法:本实验采用病例-对照研究方法对 xrcc3基因多态性与卵巢癌的易感性进行研究。采用聚合酶链反应-限制性片段长度多态性(PCR—RFLP)方法检测157 例上皮性卵巢癌患者和205例健康对照的XRCC3 A17893G SNP的基因型。统计分析应用SPSS11．5软件包。结果:1卵巢癌组和对照组的均衡性检验结果显示:健康对照组与病例组年龄、初潮年龄、孕次、产次构成无显著差异(P>0．05), 说明病例组与对照组具有良好的可比性。2 XRCC3基因 A17893G多态与卵巢癌发病风险之间的关系:(1)卵巢癌及对照组的A／A、A／G、G／G基因型频率分别为38．9％、 45．2％、15．9％和50．2％、43．4％、6．3％,两组间基因型频率分布有显著性差异(P<0．05)。(2)卵巢癌及对照组的A、 G等位基因频率分别为61．5％、38．5％及72．0％、28．0％,两组比较有显著性差异(P<0．05)。(3)与A／A基因型相比, 携带G等位基因可明显增加卵巢癌的发病风险(经年龄、性别校正OR值为1．608,95％CI为1．176—2．200)。3 XRCC3 基因A17893G多态与卵巢癌病理类型之间的关系:将卵巢癌组按照病理类型分组,发现宫内膜样癌和低分化腺癌在 XRCC3多态位点基因型分布有显著性差异(P分别为0．028、 0．030),其它病理类型多态位点基因型频率分布均无显著性差异(P>0．05)。与AA基因型相比,GG基因型可明显增加宫内膜样癌和低分化腺癌的发病风险(经年龄、性别校正OR 值分别为3．962和5．282,95％C1分别为1．477～10．623和 1．618～17．244)。4 XRCC3基因A17893G多态与卵巢癌临床分期之间的关系:将卵巢癌按FIGO分期分组后,将卵巢癌分为I、Ⅱ、Ⅲ、Ⅳ期,将I、Ⅱ期定义为早期,Ⅲ、Ⅳ期定义为晚期,XRCC3基因A17893G多态:早期卵巢癌与晚期卵巢癌两组基因型分布没有显著性差异(P>0．05)。结论:1 XRCC3基因A17893G基因型多态与中国汉族女性人群上皮性卵巢癌的易感性有关。2将卵巢癌患者按病理类型分组后,发现GG基因型可明显增加宫内膜样癌和低分化腺癌型卵巢癌的发病风险,而与浆液性卵巢癌与黏液性卵巢癌卵巢癌的发病风险无关。3将卵巢癌根据FIGO分期分组后, 发现XRCC3基因A17893G基因型多态在早期卵巢癌与晚期卵巢癌的分布存在无显著性差异。更多还原

【Abstract】 Objective: Ovarian cancer is frequency in gynecological tumour, more than 90% comes from epithelial tissue. XRCC3( X-ray repair cross complementing group 3),one member of the RAD51 gene family, which involves in homologous recombination repair(HRR), is important in keeping the integrity of the genomic activity and repairing the lesions that mutagens resulted in Single nucleotide polymorphisms (SNP) in XRCC3 gene may modify DNA repair capacity and genetic susceptibility to cancer. This study was designed to investigate the association of XRCC3 A17893G SNP with susceptibility to epithelial ovarian cancer patients in north China. Methods: Five ml of venous blood from each subjecr was drawn from 157 ovarian cancer patients and 205 healthy control subjects. The genomic DNA was extracted by using pro-teinase K digestion followed by a salting out procedure. Statistical analysis was performed using the SPSS11.5 software package. Results: 1 The general information in ovarian cancer patients was comparable to the healthy controls. 2 XRCC3A17893G: genotype frequent and aUelotype frequency in ovarian cancer patients are more than healthy controls (P>0.05). comparable with A/A genotype, individuals with G allele had higher risk to develop ovarian cancer(age and gender adjusted OR=1.608, 95%CI=1. 176 - 2.200). 3 When the epithelial ovarian cancer patients were grouped according to the pathological characteristics, significant difference were observed among the patients with serious cystadenocarcinoma or endometrioid carcinoma and the control for XRCC3A17893G SNP(P>0.05) but not in serosity ovarian cancer(P<0.05). 4 When the epithelial ovarian cancer patients were divided into two groups according to FIGO standard, there was no difference between the early group and the late group for XRCC3 (P>0.05). Conclusion: 1 XRCC3 polymorphism may be associated with susceptibility to ovarian cancer. Stratification analysis by pathologic type, G/G genotype may modify the liability to serious cystadenocarcinoma or endometrioid carcinoma By FIGO, XRCC3 may have no difference between the early group and the late group with ovarien cancer.更多还原