【作者】 耿敬姝； 薛英威； 刘秀华； 刘立仁； 高晓敏； 赵家宏；

【Author】 Geng Jingshu Xue Yingwei Lui Xiuhua Liu Liren Gao Xianmin Zhao Jiahong, Depart. of Pathology, Tumor Hospital, Harbin Medical University

【机构】 哈尔滨医科大学第三临床医学院；

【摘要】 应用流式细胞术(FCM)对64例大肠癌的石蜡包埋组织的DNA指数(DI)、S期细胞百分比(SPF)、细胞增殖指数(PI)进行检测．并且对上述三项指标与大肠癌的组织类型、组织分化程度、侵犯深度、转移及预后等临床病理因素的关系进行探讨．结果显示:在癌组织分化低的大肠癌组中,上述三项指标均高于高一中分化腺癌组,且具有显著意义(P<O．05,P<0．01)。粘液腺癌的 SPF、PI值均高于单纯腺癌组(P<O．01)。在有淋巴结转移的大肠癌中DI增高(P<O．05)。在有血行转移的大肠癌中DI及SPF、PI增高(P<O．05),P<0．01)。表明用石蜡包埋组织进行DNA倍体及细胞周期分析的回顾性研究,能反应肿瘤的生物学特性,为癌细胞群体的增殖活性与病理因素之间相关性的研究提供依据。更多还原

【Abstract】 The 64cases of cancer colon of paraffin samples were studied by Flow Cytome try (FCM) to measure DNA Index (DI), Synthetic phase fraction (SPF), Proliferate Index (PI) and to study the relationship between DI, SPF, PI and type of histology, degree of differentia tion, depth of infiltration, metastases in cancer colon. Results: DI,SPF,PI in low differentiation of cancer colon were significantly higher than that in middle and high differentiation (P＜0.05, P ＜0.01). SPF, PI in mucous adenocarinoma were significantly higher than that simple adenocaro einoma (P＜0.01). DI in lymph nodes with metastasis were higher than that in the lymph nodes without metastasis (P＜0.05). DI and SPF, PI were high in blood with metastasis (P＜0.05, P＜ 0.01). Conclusion: the results suggest that the bilogical property of tumor is responded by re view study to DNA ploidy and cell cycle with paraffin samples. It provides basis for research the relationship between proliferative activity of cancer cell and pathological factors.更多还原