【作者】 张丽娜； 葛慎光； 于京华； 朱元娜；

【Author】 ZHANG Li-na GE Shen-guang YU Jing-hua ZHU Yuan-na School of Chemistry and Chemical Engineering,University of Jinan,Jinan 250022,China

【机构】 济南大学化学化工学院；

【摘要】 研究了3-对甲苯基-5-（2′-磺酸基苯偶氮）若丹宁（4MRASP）-牛血清白蛋白（BSA）体系的共振瑞利散射光谱,在pH 2.8的B-R缓冲溶液中,SDS微乳液存在下,4MRASP和BSA的共振瑞利散射（RRS）十分微弱。当两者形成复合物时能使RRS信号急剧增强,在380～400 nm范围内呈现较高的散射强度,其最大散射波长为391 nm。4MRASP测定BSA的线性范围是0～1.4μg·mL^-1,该方法的检出限为1.78×10^-7g·mL^-1,该方法用于奶粉中牛血清白蛋白的测定,结果令人满意,并对散射增强的原因进行了解释。更多还原

【Abstract】 In this paper,the system of resonance rayleigh light scattering of the 3-（4’-methylphenyl）-5-（2’-sulfophenylazo） rho- danine and BSA was studied.At pH 2.8,under the medium of microemulsion,the interaction of 3-（4’-methyiphenyl）-5-（2’-sul- fophenylazo） rhodanine with protein results in enhanced resonance rayleigh light scattering signals at 391 nm.The linear ranges of the calibration curves is 0-1.4μg·mL^-1,and the detection limit is 1.78×10^-7g·mL^-1.The method has been applied to the analysis of protein in milk samples and the results are satisfactory.更多还原