【作者】 范文秀； 黄建华； 李新峥； 陶建中；

【Author】 FAN Wen-xiu HUANG Jian-hua LI Xin-zheng TAO Jian-zhong School of Chemistry and Chemical Engineering,Henan Institute of Science and Technology,Xinxiang,Henan,453003,China

【机构】 河南科技学院化学化工学院；

【摘要】 采用热水浸提和乙醇沉淀的方法提取南瓜多糖,Sevage法除蛋白,活性炭脱色,对南瓜多糖的理化性质、提取率、含量、组成和结构进行了研究,南瓜多糖呈灰白色粉末,溶于水,不溶于有机溶剂。碘-碘化钾反应呈阴性,说明提取物为非淀粉性多糖。提取率5.34%,总糖含量为97.93%,紫外光谱扫描结果表明南瓜多糖几乎不含核酸和蛋白质;红外吸收光谱检测表明,在3 433,2 949,1 749,1 612,1 416,1 333,1 238,1 147,1 101,1 018,833,763,637,536,427 cm^-1处表现为典型的多糖特征吸收峰;南瓜多糖是一种酸性多糖,同时存在呋喃环和吡喃环,该多糖是以α-型糖苷键相连结的杂多糖。更多还原

【Abstract】 Pumpkin polysaccharide was extracted with hot-water,sedimentated with ethanol and decolourized by active carbon. The protein in crude polysaccharide was removed by Sevage method.The physical and chemical character,extraction rate,con- tent,compositions of pumpkin polysaccharide were studied.Pumpkin polysaccharide was ashen powder,water-soluble,insolu- ble in organic solvents.The negative reaction with iodide-potassium iodine show that pumpkin polysaccharide was non-starch polysaccharide.Extraction rate was 5.34% and the content of polysaccharide was 97.93%.UV spectrum showed that there was little DNA and protein.Typical absorption peaks of infrared spectroscopy at 3 433,2 949,1 749,1 612,1 416,1 333,1 238, 1 147,1 101,1 018,833,763,637,536 and 427 cm^-1 indicated pumpkin polysaccharide is linked to the heteropolysaccharide byα-glycosidic bond.It is acid polysaccharide and comprises furan and pyran parts.更多还原