【作者】 李楠； 阎宏涛； 郭艳丽；

【Author】 LI Nan YAN Hong-tao GUO Yan-li Department of Chemistry in Northwest University,Xian 710069,China

【机构】 西北大学化学系；

【摘要】 基于在碱性条件下,Cu²⁺与锌试剂反应生成稳定的蓝色配合物,研究建立了散射背景下测定中药中痕量Cu²⁺的激光热透镜光谱分析方法。进行了明胶和表面活性剂等对该显色体系的增强效应研究。发现样品Cu²⁺和Zn²⁺共存时,加入一定量的明胶,可以消除Zn²⁺对于痕量Cu²⁺测定的干扰。在散射背景下,以激光热头镜光谱法进行了中药样品桑寄生枝叶、花生红衣中痕量Cu²⁺含量测定。Cu²⁺含量在0～1.0μg·mL^-1浓度范围内线性良好,相关系数r为0.998 4,最低检测限2 ng·mL^-1。与分光光度法、原子吸收光谱法测定结果进行对照,结果表明分光光度法受散射干扰严重,测定结果误差较大。而采用激光热透镜光谱分析法能够较好地避免散射的影响,准确地测定待测物的含量,与原子吸收法测定测定结果基本一致。更多还原

【Abstract】 A method for the determination of Cu²⁺ in scattering solutions by laser thermal lens spectrometry was presented in the paper,based on Cu²⁺ complex with Zincon in a basic medium （pH 8.0）.Gelatin can be used to improve the selectivity of the method.Comparing the results from laser thermal lens spectrometry and spectrophotometry,the effect of scattering caused by the presence of colloid in the solution was investigated.A calibration curve was found to be linear over the range of 0 to 1.0μg·mL^-1 of Cu²⁺ with the correlation coefficient of 0.998 4 and the limit of detection of 2 ng·mL^-1.The results show that laser thermal lens technique is effective for the determination of Cu²⁺ in Chinese herbal medicine.更多还原