【作者】 孙舒婷； 赵燕芳； 李茹； 韩颜颜； 李燕； 魏琴；

【Author】 SUN Shu-ting ZHAO Yan-fang LI Ru HAN Yan-yan LI Yan WEI Qin School of Chemistry and Chemical Engineering of University of Ji’nan,Ji’nan 250022,China

【机构】 济南大学化学化工学院；

【摘要】 基于依诺沙星对四-（3-甲氧基-4-羟基苯基）卟啉-铽（香兰素铽卟啉）配合物的荧光增强性能,建立了间接荧光法测定依诺沙星的体系。在pH 5.70的HAc-NaAc缓冲液及AOT微乳液环境中,香兰素铽卟啉配合物的激发与发射波长分别位于421与650 nm,依诺沙星能够有效地增强其荧光强度。依诺沙星浓度在1.00～12.00μg·mL^-1范围内与荧光强度呈良好的线性关系,检出限为0.315μg·mL^-1。本方法灵敏度高,选择性好,用于药剂中依诺沙星的测定,获得满意结果。同时采用等摩尔比法测定了依诺沙星与卟啉配合物的结合常数,初步探讨了反应机理。更多还原

【Abstract】 Based on the fluorescence enhancement of meso-（3-methoxy-4-hydroxyphenyl）porphyrin by enoxacin,a new method for the determination of enoxacin was established.At pH 5.70 and in the preseence of AOT micro-emulsion,the excitation and emission wavelength were set at 421 and 650 nm,and enoxacin can enhance its fluorescence intensity effectively.The reaction conditions of the system are studied based on series of experiments.The liner range of working curves was 1.00-12.00μg·mL^-1 for enoxacin,and the limit of detection is 0.315μg·mL^-1.The method is sensitive,stable and relatively free from inter- ferenee of co-existing species.The binding number was also studied by molar ratio method,the stable complex of porphyrin and enoxacin was formed on the molar ration of 1:3.The analytical results of market enoxacin powder injection are satisfactory.更多还原