【作者】 乔新歌； 黄成； 银亚冰； 杨新林；

【Author】 QIAO Xin-ge HUANG Cheng YING Ya-bin YANG Xin-lin (School of Life Science and Technology,Beijing institute of technology,Beijing 100081)

【机构】 北京理工大学生命科学与技术学院；

【摘要】 利用荧光显微镜研究了在显微镜汞灯绿光激发下二加成亚甲基富勒烯 [60]二膦酸四乙酯（BFDP）、二加成富勒烯[60]丙二酸（DMAC60）和富勒醇（Ful- lerol）3种不同结构富勒烯衍生物对 HeLa 细胞细胞膜的光诱导损伤作用,发现 BFDP、DMAC₆₀对 HeLa 细胞细胞膜的光诱导损伤过程在低浓度下（4μm/L）即可发生,且有浓度依赖性,而富勒醇（Fullerol）在相同光照条件下并不引起细胞膜损伤。对机理初步研究发现 BFDP、DMAC60光诱导膜损伤过程有活性氧的参与,但涉及活性氧的种类不同。这些结果直接证实细胞膜是 C₆₀衍生物的光敏作用靶点,并提示活性氧可能参与这一过程。更多还原

【Abstract】 By using fluorescence microscope,the photo-induced damage to cell membrane of HeLa cells by bisadduct of tetraethyl methano [60] fullerenediphosphonate （BFDP）,dimalonic acid C₆₀ (DMAC₆₀) and Fullerol was investigated in the presence of green light of mercury lamp.The data showed that the photo-induced toxicity against cell membrane activity occuned even at the presence of low concentration of （4 μm/L）,and the damage was dose-dependent, but the Fulllerol can not induce any damage to cell membrane of HeLa cells at the same conditions.The results demonstrated that photo-induced cell membrane damage of BFDP and DMAC₆₀were related to the produce of reactive oxygen species,and involved different species.It is concluded that cell membrane was a target of photosensitive action produced by BFDP and DMAC₆₀,and implied that reactive oxygen species was probably involved in the photosensitive action.更多还原