【作者】 曲喜龙； 刘平； 王赪胤； 朱霞石； 冷宗周； 杨功俊；

【Author】 QU Xi-long, LIU Ping, WANG Cheng-yin, ZHU Xia-shi, LENG Zong-zhou, YANG Gong-jun College of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, China

【机构】 扬州大学化学化工学院；

【摘要】 提出了测定加替沙星的紫外分光光度法和荧光光谱法,并对影响加替沙星紫外和荧光性质的不同因素进行了研究,发现在pH为4．3的缓冲溶液中,十二烷基硫酸纳(SDS)对加替沙星荧光有较强的增敏作用。当采用紫外分光光度法测定加替沙星,其吸光度与浓度在1．0×10-7～6．0×10-5mol·L-1范围内呈线性关系良好,检测限为3．5×10-8mol·L-1;而采用荧光分光光度法进行测定时,其荧光强度与浓度在1．0 ×10-9～3．0×10-6mol·L-1范围内呈良好线性关系,检测限为4．0×10-10mol·L-1。并对加替沙星胶囊和血清样品中的加替沙星含量和回收率进行了测定,获得满意结果。更多还原

【Abstract】 UV-Vis spectrophotometric and spectrofluorimetric methods for the determination of gatifloxacin were proposed, and the main factors affecting on UV-Vis and fluorescence of GFLX were studied. It was found that the addition of an appropriate amount of sodium dodecyl sulfate (SDS) to the solution of gatifloxacin in HAc-NaAc buffer solution (pH 4. 5) led to a remarkable enhancement of the fluorescence intensity of gatifloxacin. The calibration curve was linear in the ranges of 1. 0×10-7-6. 0× 10~5 mol · L-1 with the detection limit of 3. 5 × 10~8 mol · L-1 for gatifloxacin by UV-Vis method and 1. 0× 10-9-3. 0× 10-6 mol · L-1 with the detection limit of 4. 0× 10-10 mol · L-1. The recommended methods were applied to determine the content of gatifloxacin and the recoveries in gatifloxacin capsules and in human serum with satisfactory results.更多还原