【作者】 黄晓梅； 洪碧琴； 刘琴英； 林萍萍； 曾小毅； 刘佳铭；

【Author】 Xiao-Mei Huang (Department of Food and Biological Engineering, Zhangzhou Institute of Technology, 363000, Zhangzhou); Bi-Qin Hong, Qin-Ying Liu, Ping-Ping Lin, Xiao-Yi Zeng, Jia Ming Liu* (Department of Chemistry, Zhangzhou Normal College)

【机构】 漳州职业技术学院； 漳州师范学院化学系；

【Abstract】 A new method using 1.5-generation dendrimers molecule to spike fluorescein to label triticum vulgare lectin affinity adsorption solid substrate-room temperature phosphorescence for the determination of sugar has been established. The detection limit of this method calculated by 3Sb/k is 0.021 pg spot–1 (5.2×10–11 g mL–1). Compared with the direct single antigen-antibody method. This method has been successfully applied to the determination of sugar in human serum, as well as the survey and forecast of human diseases, for it is of good sensitivity, accuracy and precision.更多还原