【作者】 彭淑鸽； 张玉清； 高秋明；

【Author】 Shu-Ge Peng*a, Yu-Qing Zhang a, Qiu-Ming Gaob a Key Lab of Polymer and Nanomaterials, University of Science and Technology of Henan, Luoyang, 471003

【机构】 河南科技大学高分子与纳米材料重点实验室； 中国科学院上海硅酸盐研究所高性能陶瓷和超微结构国家重点实验室；

【Abstract】 Based on the obtained exfoliated magadiite, Layered nanocomposites of Mb- and Hb-magadiite have been successfully prepared under mild conditions. The enzyme activities of the bio-inorganic compsites were studied under different substrates. The results showed immobilized heme proteins had enhanced enzyme activity when o-phenylenediamine was used as substrate. There were nearly 5 and 8 times increases in Vmax and kcat values for the immobilized Mb and Hb compared to that of free heme proteins, respectively, based on the Lineweaver-Burk plots of enzymatic activity parameters’ analyses. The high catalytic activity might be that the immobilized heme proteins had the more proper conformation for o-phenylenediamine than that of free heme proteins. The high catalytic activity would endue the immobilized heme proteins with potential commercial application in biosensors and biocatalysts.更多还原