【作者】 户敏； 沈鹤柏； 王超； 董亚明；

【Author】 Chao Wang, He Bai Shen, Min Hu,Yaming Dong Life and Environment Science College, Shanghai Normal University, 200234, Shanghai

【机构】 上海师范大学生命与环境科学学院；

【Abstract】 Polymerase chain reaction(PCR), a technique for in vitro amplification of a DNA fragment, is one of the most important experimental methods in molecular biology.PCR and asymmetric PCR process based on nanoparticle have been studied by our groups systemicly.However, the mechanism of the PCR process based on nanoparticle is still remain unknown. In this paper, we make modified primers covalently attached to surface of the Au nanoparticles and the magnetic nanoparticles,and research the regulation with the PCR cycles . Atomic Force Microscopy (AFM) was adopted to detect the PCR products.更多还原