【作者】 杨景峰； 王思珍； 董武；

【Author】 Jingfeng Yang, Sizhen Wang, Wu Dong (Animal science and Technology College, Inner Mongolia University for Nationalist. Tongliao 028042, China)

【机构】 内蒙古民族大学动物科学技术学院；

【摘要】 二噁英为主的POPs物质(Persistent Organic Pollutants,持久性残留性有机污染物质) 是环境中具有高残留性、广泛分布性和高生物毒性的物质。本实验使用其中毒性最强的环境污染物质2,3,7,8-四氯-二苯基-对-二(?)英(2,3,7,8-tetrachlorodibenzo-p-dioxin,以下略为TCDD)作为标准对斑马鱼胚胎进行了染毒。通过原位杂交技术检测到0．1μg/L,0．3μg／L,0．5μg／L,1．0μg／L 的TCDD在鳃弓部的CYP1A1 mRNA表达量存在明了的浓度-剂量关系,进一步通过免疫学反应, 检测出的CYP1A1蛋白质表达亦存在清晰的浓度-剂量关系。这说明用CYP1A1在鳃弓部位的表达强度以及结合形态学进行超微量二(?)英污染物质的生物学评价具有一定的可行性。更多还原

【Abstract】 Persistent organic pollutants (POPs) including the Dioxin family are chemicals that remain intact in the environment for long periods, become widely distributed geographically, accumulate in the fatty tissue of living organisms and are toxic to humans and wildlife. We found the dose-dependent expression of CYP1A mRNA and CYP1A protein expression by the in situ hybridization and immunohistochemistry staining after exposed TCDD (0.1 μg/L, 0.3 μg/L, 0.5 μg/L and 1 μg/L) in the gill arch of zebrafish embryos. This result indicated that it is a possible for bioassess of dioxin by the intensity of CYP1A1 express with morphological.更多还原