【作者】 苏燕； 任磊； 罗义； 姜旭东； 王晓蓉；

【Author】 SU Yan, REN Lei, LUO Yi, JIANG xu-dong, WANG Xiao-rong (State Key Laboratory of Pollution Control and Resources Reuse,School of Environment Science ,Nanjing University, Nanjing210093, China)

【机构】 南京大学环境学院污染控制与资源化研究国家重点实验室；

【摘要】 采用实验室模拟的方法,将鲫鱼(Carassius auratus)分别暴露于五氯酚(PCP)、2,4, 6-三氯酚(2,4,6-TCP)10d后,运用SDS-PAGE和Westrn Blotting方法检测鱼脑组织内应激蛋白 HSP70的诱导表达情况。结果表明:在整个实验浓度范围内,随着PCP和2,4,6-TCP浓度的增加, HSP70的相对灰度值都呈现先诱导后抑止的大体趋势;HSP70对PCP暴露更为敏感,在0．001mg／l (低于国家渔业水质标准)时对鱼脑HSP70有较显著诱导(p<0．005),充分说明运用分子生物学指标要比传统的检测指标更敏感,可以考虑HSP70作为两种氯酚污染的生物标志物。更多还原

【Abstract】 After the fish(Carassius auratus) were exposed to PCP and 2,4,6-TCP respectively for ten days, a laboratory experiment was carried out to detect the expression level of HSP70 in the fish brain using SDS-PAGE and Western Blotting methods.The results showed that within the overall dose range in the experiment, the induction of HSP70 was firstly induced and then prohibited as the concentrations of PCP and 2,4,6-TCP increased;PCP is a much stronger inducer of HSP70 than 2,4,6-TCP that even at the dose of 0.001mg/l(below the criterion of national piscatorial water quality) PCP could induce HSP70 intensively.更多还原