【作者】 徐向东； 胡涌刚； 李欣欣；

【Author】 XU Xiangdong, HU Yonggang, LI Xinxin (Institute of Environmental Science & Engineering; Department of Chemistry, Huazhong University of Science and Technology,Wuhan 430074)

【机构】 华中科技大学环境科学与工程学院；

【摘要】 某些氨基酸和蛋白质在碱性条件下具有能显著增强鲁米诺-铁氰化钾体系化学发光信号的特性,本文以L-精氨酸和胃蛋白酶为分析对象,对鲁米诺-铁氰化钾体系用于毛细管电泳-化学发光法直接分离氨基酸和蛋白质的可行性进行了研究,并优化了化学发光和电泳条件。在优化条件下,L- 精氨酸和胃蛋白酶可直接实现分离,其检测限分别为1．3 × 10-4 mol／L和7．1 × 10-7 mol／L。该方法无需复杂的衍生步骤,操作简便,应用于实际生物样品的分析测定,结果令人满意。更多还原

【Abstract】 A novel method for the analysis of amino acid and protein was developed by capillary zone electrophofesis -chemiluminescence (CZE-CL) method based on the fact that some amino acids and proteins could catalyze the chemiluminescence reaction of Luminol-K3[Fe(CN)6] system. In addition, 2.5mmol/L sodium tetraborate-2×10-3mol/L luminol and 2×10-4 mol/L potassium ferricyanide (pH=13.3) were chosen as optimum separation and detection condition for the analysis of L-arginine and pepsin .The detection limits (S/N=3) for L-arginine and pepsin were 1.3×10-4mol/L and 7.1×10-7mol/L,respectively. Finally, the presented method was used for the analysis of three real L-Arginine samples successfully.更多还原