【作者】 罗云敬； 林太凤； 李芸； 张伟； 钟儒刚；

【机构】 北京工业大学生命科学与生物工程学院；

【摘要】 <正>过亚硝酸根(ONOO-)是存在于生物系统中的强活性氧化剂,能够修饰DNA碱基、造成DNA单链断裂,并且能够修饰氨基酸残基及蛋白质等生物大分子。过亚硝酸根对生物大分子的损伤有可能导致多种疾病如冠心病、动脉硬化和肾衰竭等,所以研究过亚硝酸根对生物大分子的损伤以及建立合适的分析方法具有重要意义。本文利用仪器分析手段,建立了在生理条件(pH 7．4)下研究 ONOO-损伤脱氧鸟苷(dG)、酪氨酸、色氨酸的分析更多还原

【Abstract】 Peroxynitrite is a powerful oxidant in vivo. Peroxynitrite can damage biomacromolecules, which may lead to many diseases such as coronary heart disease, atherosclerosis, renal failure and so on. It is of a great significance to investigate biomacromolecules damage by peroxynitrite and to establish suitable analytical methods. In this paper, three analytical methods under physiological pH have been established to study dG modification, tyrosine nitration, tryptophan damage induced by peroxynitrite. With HPLC-EC, dG oxidative damage by peroxynitrite has been studied; fluorescence method was established for the study on tryptophan reacted with per-oxynitrity; the amount of 3-nitrotyroine produced from the reaction of tyrosine with peroxynitrite was measured by UV spectrophotometry.更多还原