诺氟沙星-碘荷移络合物荧光光谱的研究与应用

【作者】 张敏； 曹玺珉； 杜黎明；

【机构】 山西师范大学分析测试中心；

【摘要】 荷移荧光光谱法由于具有高灵敏度和高选择性而受到广泛关注。而采用无机物作为荷移试剂的荧光荷移反应研究尚未见报道。本文采用荧光分光光度法研究了诺氟沙星(NOR)与碘的荷移络合物,优选了荷移苗合物的形成条件,发现在无水乙醇中易生成稳定的络合物,并能使NOR的荧光光谱得到显著增强。据此建立了高灵敏度测定NOR的荧光光谱新方法。该方法简便、准确、选择性好,用于药物制剂中NOR含量的测定。更多还原

【Abstract】 Iodine-norfloxacin charge-transfer complexes are demonstrated to enhance the fluorescence of norfloxa- cin. Thus the simple and reliable fluorescence spectro-metric method for the determination of NOR has been developed based on the charge transfer reaction. The linear calibration graphs was obtained over the NOR concentration range of 0. 005 ~ 4. 0 mg/L. The proposed procedures could be applied successfully for the determination of the investigated drugs in their pharmaceutical preparations with good recovery; percent ranged (%) from 98.09±0.90 to 99.97 ±0.99.更多还原