【作者】 吕爱星； 卢春华； 邱彬； 陈国南；

【Author】 LV Ai-xing, LU Chun-hua, QIU Bin and CHEN Guo-nan (Department of Chemistry, Fuzhou University, Fuzhou 350002)

【机构】 福州大学化学化工学院化学系；

【摘要】 采用荧光淬灭法对奈韦拉平的测定方法进行研究。采用新型荧光试剂, 在激发波长为225 nm,发射波长为340 nm,pH=6．0的磷酸盐缓冲介质中,荧光试剂的荧光淬灭程度与奈韦拉平(NVP)浓度之间有着良好的线性关系,奈韦拉平的线性范围为5．0×10-7-1．0×10-5mol／L,检测限为1．0×10-7mol／L,相关系数为0．9946,方法具有杂质干扰少,操作简便等优点。更多还原

【Abstract】 To determine the content of nevirapine, a fluorescence quenching method has been developed. The excitation wavelength was 225 nm and emission wavelength was 340 nm. The calibration curve was linear over the range of 5.0 × 10-7 ~ 1.0 × 10~3 mol/L. Cor-relationco efficient was 0. 9946. The recovery of NVP was 102% (n = 5) and the relativestan darddeviation (RSD) was 2.4%. The RSD for intraday and interday were2.6% (n=5) and3.8% (n = 5), respectively.更多还原