【作者】 康天放； 闫骏； 高慧丽；

【Author】 Kang Tian-Fang Yan Jun Gao Hui-LiCollege of Environmental and Energy Engineering, Beijing University of Technology, Beijing100022

【机构】 北京工业大学环境与能源工程学院；

【摘要】 通过交联方法将胆碱氧化酶直接固定于铂盘电极表面,用戊二醛熏蒸法固定乙酰胆碱酯酶于Pall膜上,再将该膜固定于胆碱氧化酶膜表面,制成双酶电化学生物传感器.优化了制备生物传感器的实验条件.百分抑制率与对硫磷浓度的对数值之间在对硫磷浓度为1.0×10-8g/L到1.0×10-4g/L之间存在线性关系,检测限为2.4×10-9g/L.更多还原

【Abstract】 Choline oxidase was first immobilized on the surface of a platinum disc electrode by cross-linking method. A Pall membrane drop-covered with a layer of acetylcholinesterase was exposed to the vapor of glutaraldehyde in order to immobilize acetylcholinesterase on the surface of the membrane. Then the Pall membrane was placed on the choline oxidase layer to make bienzymatic electrochemical biosensor. The experimental conditions of preparing the biosensor were optimized. The percent inhibition was proportional to the logarithm of parathion concentration over the range of 1.0×10-8 g/L to 1.0×10-4g/L with a detection limit of 2.4×10-9 g/L.更多还原