【作者】 温锡敏； 张进平； 黄莉； 乔旭；

【Author】 Wen Ximin Zhang Jinping Huang Li Qiao Xu(College of Chemistiyald Chemical Engineering Nanjing University of Technology,Nanjing 210009)

【机构】 南京工业大学化学化工学院；

【摘要】 针对以苯甲醛、氯仿为原料合成苯甘氨酸的反应过程,建立了高效液相色谱法测定苯甘氨酸含量的方法,考察了流动相组成与流量对分离效果的影响,得出了适宜的分析条件。选择10%甲醇+90%磷酸二氢钠溶液(20 mmol/L)为流动相,在其流量1 mL/min、柱温为室温和检测波长为260 nm的条件下,苯甘氨酸浓度在25-400 mg/L范围内,浓度与峰面积呈较好的线性关系,r=0.999 9,系统精密度RSD=0.13%。更多还原

【Abstract】 For the process of phenylglycine synthesized from benzaldehyde and chloroform, the alialysis method of phenylslycine by HPLC was established. The influence of composition and flow - rate of mobile phase on the separation degree was examined. The optimized chromatography conditions are as follows: methanol/monosodium phosphate(20 mmol/L) = 10/90(v/v) as the mobile phase,flow- rate of mobile phase/1 mL/min, wavelength of detection 260 nm. The standard curve for phenylglycine was linear over the range of 25 - 400 mg/L, r = 0.999 9. The accuracy of system was RSD = 0.13%.更多还原