【作者】 何毛贤； 林岳光； 胡建兴； 沈琪； 姜卫国；

【Author】 He Maoxian Lin Yueguang Hu Jianxin Shen Qi Jiang Weiguo (South China Sea Institute of Oceanology,Chinese Academy of Sciences,Guangzhou 510301)

【机构】 中国科学院南海海洋研究所；

【摘要】 一种新的诱导剂6-二甲氨基嘌呤（6-DMAP）抑制第二极体诱导合浦珠母贝 Pinctada martensii（D．）三倍体。对6-DMAP 浓度,处理持续时间及起始时间进行了研究。结果表明孵化率与这三个参数呈负相关,而三倍体诱导率在一定范围内呈正相关。合适的6-DMAP 浓度为60～80mg．L^-1,处理持续时间约20min,起始时间的选择受水温及卵发育程度的影响。75mg．L^-1 6-DMAP 与0．5mg．L^-1细胞松驰素 B （CB）处理对比,6-DMAP 处理组的孵化率（4．57%）明显高于 CB 处理组（2．23%）,而三倍体诱导率（90．00%）与 CB 处理组（93．55%）差别不明显。由于6-DMAP 具有低毒、高效、易于操作的特点,是一种优良的多倍体诱导剂。更多还原

【Abstract】 In this study,6-dimethylaminopurine（6-DMAP）,a new inductor,was used to inhibit the sec- ond polar body in order to induce triploid pinctada martensii （D.）.The effects of 6-DMAP concen- tration,treatment duration and timing of treatment after fertilization on the normal D-shaped larvae ratio and the percentage of triploids were examined.The results indicated that the survival to D- stage was inversely related to 6-DMAP concentration,treatment duration and time of treatment after fertilization;and that the percentage of triploids was related to these three parmeters.Optimal 6-DMAP concentration was 60～80 mg.L^-1.Optimal treatment duration was about 20 min.The timing of treatment application was dependent on the temperature the of seawater and the develop- mental condition of the Oocytes.Treatment with 75mg.L^-16-DMAP resulted in 4.57% survival rate to D-stage.The rate of survival to D-stage was greater than 2.23% for the group treated with 0. 5mg.L^-1CB.There was no significant difference in the percentage of triploids induced by treatment with 6-DMAP （90.00%） with 6-DMAP（90.00%）,or with CB （93.55%）.6-DMAP treatment is a new method that is easy to handle,and is a less dangerous,most efficient technique to induce triploid Pinctada martensii （D.）.更多还原