【作者】 翟琳娜； 张思祥； 尹霞； 冉多钢； 刘伟玲；

【Author】 ZHAI Linna ZHANG Sixiang YIn Xia RAN Duogang LIU WellingResearch Institute of Modern Measurement & Control Technology Hebei University of Technology, Tianjin, 300130

【机构】 河北工业大学现代测控技术研究所；

【摘要】 随着生命科学研究和临床医学诊断的发展,要求荧光显微镜不仅能满足主观的观察,而且要求具有计算机客观分析;不仅能提供图像形态信息,而且要提供图像光谱信息。本系统研究就是为了满足这一要求而提出的,解决了传统荧光显微镜不能提供连续可调的激发光谱、无法获得数字化荧光显微图像信息和光谱信息等问题。本论文对显微荧光图像连续光谱分析系统的构成的关键技术:激发光源部分、荧光图像采集部分和计算机控制分析部分进行了详细地叙述。更多还原

【Abstract】 With the development of the research of life sciences and clinic medicine diagnose, the fluorescence microscope is requested to not only satisfy subjective observation, but also have computer external analysis. It also is requested to not only offer image conformation information, but also offer image spectrum information. The research of the system is brought forward for satisfying the requestions. It found the solutions to that the traditional fluorescence microscope could not offer contiguous adjustable excitation spectrum, and that we could not get digitized fluorescence microscopic image information and spectrum information and so on. The paper detailedly specifies the pivotal technologies of the research of the continuous spectrum analytic system of micro-fluorescence image: the part of excitation lamp-house, the part of micro-fluorescence image acquisition and the part of computer control analysis.更多还原