【作者】 姚晋丽； 王霞； 金伟其； 陈华；

【Author】 YAOJinli WANG Xia JIN Weiqi CHEN Hua SUBinghua(Dept of Optical Engineering, School of Information Science & Technology, Beijing Institute of Technology, Beijing, 100081)

【机构】 北京理工大学信息科学技术学院光电工程系；

【摘要】 三维显微图像复原方法通常分为两类:邻域法和三维去卷积法。本文详细介绍了邻域法的基本原理;对邻域法公式中参数c,a的不同选择和相应的复原结果进行了评价,通过仿真实验得出了考虑不同切片数时c,a的最佳选择以及不同显微成像系统所需考虑切片数M的最佳选择。与三维去卷积法相比,邻域法具有简单、计算量小,容易实现等优点,适合于观察活的生物和进行实时连续观察。更多还原

【Abstract】 The nearest neighbour algorithm and three-dimensional deconvolving algorithm are two methods of three-dimensional microscope image restoration. In this paper, firstly we introduce the fundamental of nearest neighbour algorithm carefully. Then we obtain the best choice of c,a under different slices number selection by comparing the restoration quality with different choices of c,a. And we also obtain the reasonable selection of slices number M in different microscope imaging systems. At last, through comparing three-dimensional deconvolving algorithm to nearest neighbour algorithm, we know that the nearest neighbour algorithm is simple, easy to programme and suitable to observe living samples.更多还原