【作者】 鲁强； 骆清铭； 曾绍群；

【Author】 LU Qiang, LUO Qing-ming, ZENG Shao-qun(The Key Laboratory of Biomedical Photonics of Ministry of Education, Huazhong University of Science & Technology, Wuhan, Hubei 430074, China)

【机构】 华中科技大学生物医学光子学教育部重点实验室；

【摘要】 发展了蒙特卡罗方法用于模拟混浊介质深层的单、双光子激发荧光显微成像。针对横向分辨率的研究,提出了基于点扩散函数的快速算法,由于避免了成像中扫描过程的模拟,大幅度地提高了效率。在此基础上,研究了数值孔径对单、双光子显微成像的影响。结果表明,数值孔径的增加将降低混浊介质深层显微成像的横向分辨率,但有利于提高层析能力。更多还原

【Abstract】 Monte Carlo method is enhanced to simulate single-, two-photon excitation fluorescence microscopic imaging through turbid media. Furthermore, a rapid modeling method, which is based on point spread function, is developed to study lateral resolution. Since simulation of scanning process in imaging is avoided, high efficiency is obtained. With this model, effects of numerical aperture (NA) on single- and two-photon microscopic imaging through turbid media are researched. Results show that higher NA brings lower lateral resolution but higher axial sectioning ability.更多还原