【作者】 冯庆玲； 李晓明；

【Author】 FENG Qingling, LI Xiaoming (Department of Materials Science and Engineering, Tsinghua University, Beijing 100084, China)

【机构】 清华大学材料科学与工程系；

【摘要】 制备了新颖的甲壳素纤维(CF)增强多孔胶原基骨组织工程框架材料,其成分为纳米羟基磷灰石/胶原/ 聚-L乳酸/甲壳素纤维(nHAC/PLLA/CF)。为了进一步增强支架材料,采用蛋白交联试剂N,N’-二环己基碳酰亚胺(DCC)交联CF和聚-L-乳酸(PLLA)。采用扫描电镜(SEM)观察了交联前后的框架材料,采用X-射线光电子能谱(XPS)表征了交联前后的CF,测试了框架材料的压缩强度和孔隙率。结果表明,与PLLA成功交联后的CF 能够使材料的压缩强度提高4-5倍;而在没有进行交联的情况下,CF仅能使材料的压缩强度提高2-2．5倍。交联后的材料与没有进行交联的材料相比,结构和孔隙分布更加均匀,而孔隙率没有明显的变化。所以,经过交联后的nHAC/PLLA/CF框架材料是一种很有前途的骨组织工程框架材料。更多还原

【Abstract】 In this paper, a novel porous scaffold for bone tissue engineering was prepared with nano-hydroxyapa tite/collagen/Poly-L-lactic acid (PLLA) composite reinforced by chitin fibres (CF). To enhance the strength of the scaffolds further, PLLA was linked with CF by Dicyclohexylcarbodimide (DCC). The structures of the reinforced scaffold with and without linking were characterized by scanning electron microscopy (SEM). The chemical characteristics of the CF with and without linking were evaluated by X-ray photoelectron spectroscopy (XPS). The mechanical strength was measured by compressive tests. The results suggest that after linked with PLLA successfully, the CF can reinforce the scaffolds much more effectively. The linked scaffolds, with the compressive strength 4-5 times higher than that of the unreinforced scaffolds, have also more uniform structure and pore size than that of the scaffolds without linking. So the linked reinforced scaffold is a kind of potential appropriate scaffold for bone tissue engineering.更多还原