【作者】 李效阳； 张顺利； 卢爱玲； 段瑞峰； 胡轶红； 徐存拴；

【Author】 LI Xiao-Yang ZHANG Shun-Li LU Ai-Ling DUAN Rui-Feng HU Yi-Hong Xu Cun-Shuan (Department of Biology, Henan Normal University, Xinxiang 453002, China)

【机构】 河南师范大学生物学系；

【摘要】 用免疫组织化学方法研究了大白鼠肝脏在46℃热休克30分钟后的恢复期(2.5～72小时)内HSP70的表达定位和动态变化,及不同温度(42、44和46℃)处理30分钟、恢复12小时后,HSP70的表达.结果表明:(1)HSP70主要定位于肝细胞的细胞质中;(2)在肝小叶中,阳性细胞多集中于中央静脉周围;(3)46℃处理30分钟、恢复8～12小时,HSP70表达量最多;(4)不同温度热休克处理30分钟、恢复12小时表明,46℃处理组诱导大白鼠肝细胞表达HSP70最多.用体视学方法对实验组的HSP70表达进行了定量组织学分析.更多还原

【Abstract】 The location and dynamics of HSP70 expression in rat hepar was studied with immuno-histochemistry, which were treated under different temperature (42 44 46°C) for 30 min and different recovery time (2.5-72hr). The results show: (1) In a hepatic lobule, HSP70 locates in cell plasma; (2) HSP70 is easy to beexpressed in the cells nearby centrul vein; (3) Recover for 8-12 hr after treatment of 46°C for 30min, the synthesis of HSP70 reaches climax; (4) The results of heat shock under different temperature (42 44 46°C for 30 min) and recovery (25°C for 12hr) suggest that HSP70 was induced.largely in hepatocytes at 46°C. The sterological methods was used to analysis the quantity of HSP70 expression.更多还原