【作者】 虞鹏程； 李思光； 夏菱； 黄国东；

【Author】 YU Peng-Cheng LI Si-Guang XIA Ling HUANG Guo-Dong(Department of Biological Science and Technology, Nanchang University, Nanchang 330047, China)

【机构】 南昌大学生物科学工程系；

【摘要】 以牛血清白蛋白、植物血凝素、肠型点状产气单胞菌灭活菌苗作为抗原,对荷包红鲤Cyprinus carpio Red Purse-carp的免疫性进行了初步研究.采用聚丙烯酰胺薄层等电聚焦电泳法分离未免疫与免疫后荷包红鲤血清蛋白.结果显示,同种抗原对不同PI值的血清蛋白影响各不相同,不同种抗原对相同PI值的血清蛋白也不完全相同,三种抗原对于PI值低的血清蛋均显示出“两低夹一高”的变化过程,而BSA对PI值高的血清蛋白显示出“两高夹一低”.PHA和产气胞菌灭活菌苗则示出“两低夹一高”的变化过程.同时,均在阴极端多出一条蛋白色带.更多还原

【Abstract】 This paper deals with the immune response of the wuyuan Red purse-carp (Cyprinus car-pro) to Bovine serum albumin (BSA) phytohenagg lutinin (PHA), formatin-killed vaccine of Aeromonas punctata f. intestinalis. Thin-Layer isoelectric focusing polyacryaminde gel elec-trophoresis separated serum protein in carp. The results showed that the influence of the same antigen on different serum protein was different and the influence of the different antigens on same serum protein was different too. These changes were different to the three antigers in different area. We also found that there was a new band named X in the cathoder-ay end. These findings play an important role in the research of immune respohse of fishes.更多还原