【作者】 王俊玲； 张本忠； 孙应彪； 苏莉；

【Author】 Junling Wang Benzhong Zhang Yingbiao Sun Li Su Department of Public Health, Lanzbou University, Lanzhou 730000, China

【机构】 兰州大学公共卫生学院；

【摘要】 目的研究过量氟对小鼠神经细胞的毒作用及其机制,为探讨氟中毒的发生机制提供实验依据。方法采用①体外神经细胞培养即根据加氟浓度（0、5.0、10.0、15.0、 20.0、40.0ug/ml）不同分为6组,在培养15d 时,收集各组细胞,流式细胞术检测细胞凋亡,激光扫描共聚焦显微镜,记录细胞内 Ca²⁺荧光图像;②建立氟中毒动物模型即按染氟剂量（0、0.7、2.8、11.2mg/kg）分为4组,制备细胞悬液收集脑细胞,流式细胞术检测细胞凋亡,记录细胞内 Ca²⁺荧光图像。结果体外培养神经细胞加入 NaF（≥5.0ug/ml） 和体内腹腔注射NaF（≥0.7mg/kg）均可导致神经细胞凋亡数增加和神经细胞游离钙离子数增多,与对照组比较差异均有显著性（p<0.01）。结论说明氟可诱导神经细胞调亡增加,神经细胞游离钙增加,对神经细胞具有毒作用。更多还原

【Abstract】 Objective To understand the neurotoxicity of fluoride and its mechanisms; And to search for the feasible way of preventing and curing the fluride-induced neurotoxicity. Methods ①Neuron culture in vitro. Neuron were cultured with different doses of fluoride （5.0ug/ml、10.0ug/ml、15.0ug/ml、20.0ug/ml、40.0ug/ml） . To observe morphology and recollect neuron in 15 days of cell culture. Apoptosis was detected using a flow cytometry and [Ca²⁺]i in neuron was inspected by confocal laser scanning microscopy;②Experimental in vivo. Wistar newborn rats were injected intraperitoneal with different doses of fluoride （0.7mg/kg、2.8mg/kg、11.2mg/kg） ; then apoptosis was detected using a flow cytometry and [Ca²⁺]i in neuron were inspected by confocal laser scanning microscopy. Results The number of apotosis and [Ca²⁺]i was promopted more than control group （p<0.01）.Conclusions Flouride coulde induce the apotosis and intracellular Ca²⁺ and has the neurotoxicity.更多还原