【作者】 陆小平； 肖靓； 陈正凯； 王利芬； 楼程富；

【Author】 Lu Xiaoping Xiao jing Chen Zhengkai Wang Lifen College of city sciencee, Soochow University, Suzhou 215006 Lou Chengfu College of Animal Sciences, Zhejiang University,Hangzhou 310029

【机构】 苏州大学城市科学学院； 浙江大学动物科学学院；

【摘要】 蒙古桑经-3℃诱导48h后,提取幼茎RNA反转录合成cDNA(第一链),以cDNA为模板,利用RT-PCR技术克隆桑树的泛素基因(mUb).研究结表明:克隆片段序列为泛素基因的阅读框架;其中有2 个起始密码(ATG)和1个终止密码基因(TAA);长度为459个碱基,编码152个氨基酸;序列与菠萝、三叶胶树、烟草等其它物种的泛素基因相比,有84%以上的同源性.进一步分析表明,该基因是桑树的二串泛素基因.该基因已被GenBank收录,登录号DQ104334。更多还原

【Abstract】 Mongolian Mulberry was induced 48 hours with low temperature(-3 ℃).RNA of caulicle was extracted and reversely transcvibed into cDNA .Vsing the cDNA as a templet, then Ubiquitin gene(mUB) of mulberry could be cloned by RT-PCR. The results suggest that the sequence of the cloned slice was Open Reading Frame(ORF) of ubiqutin gene,and it included two start codes(ATG),and one stop code(TAA). The slice 459 bp, encoded 152 amino acids. Compared with pineapple, Hevea, Tobacco and so on, the sequence identity was more than 84%. Further analysis suggests that this gene was PolyUb multibiqutin gene of mulberry, It has been embodied by GenBank with the acceaion No DQ104334.更多还原