【作者】 牛宝龙； 孟智启； 翁宏飙； 沈卫锋； 吕顺霖；

【Author】 Niu Baolong~(1,2) Meng Zhiqi~2 Shen Weifeng~2 Weng Hongbiao~2 Lu Shunlin~1(1 Department of Sericulture and Apiculture,College of Animal Science,Zhejiang University,Hangzhou 310029,China2 Sericulturai Research Institute,Zhejiang Academy of Agricultural Science,Hangzhou 310021,China;)

【机构】 浙江大学动物科学学院； 浙江省农业科学院蚕桑研究所；

【摘要】 利用生物信息学方法成功地克隆了家蚕磷酸甘油醛异构酶(triosephosphate isomerase Tp(?)) 基因(GenBank 登记号为AY734490),全长为2,875 bp;含有5个外显子、四个内含子,所有内含子/外显子边界都符合典型的GT/AG 剪切模式;cDNA 序列全长1,051 bp,并经RT-PCR 进行克隆、序列分析进行了验证;具有完整的开放阅读框架(ORF,95～839 bp),可编码248个氧基酸的蛋白(?)通过与NCBI 蛋白数据库的比对表明,该基因编码的蛋白为磷酸甘油醛异构酶(EC 5.3.1.1),参与葡萄糖分解代谢,即将磷酸二羟丙酮转化为3-磷酸甘油醛,在果蝇、蚊子、黄粉虫、美洲淤夜蛾、棉铃虫等昆虫中具有高度的保守性。更多还原

【Abstract】 A novel silkworm (Bombyx mori) triosephosphate isomerase(Tpi) gene is cloned byblasting its EST database and PCR test,and its sequence has been deposited in the GenBank(accession No:AY734490).It has five extrons and four introns spanning 2875 bp in full lengthand all the boundary of extrons/introns has GT/AG conserved sequences.Its 1051bp mRNA has acomplete open reading fragment (ORF:95-834) and encodes 248 Aa protein,which istriosephosphate isomerase (EC 5.3.1.1) for catalysis of conversion dihydroxyacetone phosphateinto glyceraldehydes 3-phosphate in glucose glycolysis and very conserved in many insects suchas Heliothis virescens,Helicoverpa armigera,Tenebrio molito,Anopheles gambiae and Drsophilamelanogaster.更多还原