【作者】 汤茶琴； 陈暄； 黎星辉； 房婉萍； 杨亦扬；

【Author】 TANG Cha-qin1,2, CHEN Xuan1, LI Xing-hui1, FANG Wan-ping1*, YANG Yi-yang1 (1 Tea Research Institute, Nanjing Agricultural University, Nanjing 210095, China; 2. Tea Variety Research Institute, Wuxi 214063, China)

【机构】 南京农业大学茶叶科学研究所； 无锡市茶叶品种研究所；

【摘要】 应用cDNA-AFLP(Amplified fragment length polymorphism)技术,分析了茶树在花蕾发育过程中基因表达的差异。其中一个特异片段测序结果表明,其氨基酸序列与拟蓝芥的一种蛋白激酶的同源性为77%。然后通过3’/5’RACE的方法,分别扩增出3’端和5’端的序列,从而获得茶树钙调蛋白激酶基因的cDNA全长序列,所得序列全长2282bp,编码760个氨基酸。全长cDNA序列提交GenBank,获得基因登录号EU732607。且经BLAST分析表明,该基因序列与其他植物源蛋白激酶基因的氨基酸序列同源性达到52%～89%。更多还原

【Abstract】 To obtain insights to the comprehensive molecular characteristics relating to the mechanisms of flower development of tea plant, we performed a large-scale screening of flowering related genes in flower buds by cDNA-AFLP. A cDNA encoding a protein kinase gene homolog was isolated and identified. The complete cDNA sequence was cloned by RACE (rapid amplification of cDNA ends), named Camellia sinensis protein kinase gene. The complete protein kinase gene cDNA is 2282 bp. Its cDNA sequence has been accessed to GenBank, and accession number is EU732607. Analysis of the cloned complete cDNA showed that it encompassed a open reading frame (ORF) with 2127 bp encoding 708 amino acid (aa) residues. By using the program of Blast on GenBank database, the sequence presented a high match with kinase genes from other plants, such as Arabidopsis thaliana, Brassica rapa subsp et al. we concluded that the cloned sequence was a member of kinase gene from Tea plant.更多还原