【作者】 郑红发； 黄亚辉； 黄怀生；

【Author】 ZHENG Hong-fa, HUANG Ya-hui, HUANG Huai-sheng ( Hunan Tea Research Institute, Changsha 410125, China)

【机构】 湖南省茶叶研究所；

【摘要】 对茶叶中γ-氨基丁酸的提取、分离及检测方法进行了研究,分别采用水和乙醇作为提取溶剂,结果发现用水提取能达到更好的效果,γ-氨基丁酸含量提高20％;同时采用离子交换树脂对γ-氨基丁酸提取液进行分离,提取液上732阳离子树脂柱(2．5 ×50cm),使用柠檬酸和氨水缓冲液进行pH线性梯度洗脱,洗脱液中γ-氨基丁酸最高浓度达39％;采用薄层扫描仪对γ-氨基丁酸的含量进行检测,研究并确定了检测的条件及参数。更多还原

【Abstract】 γ-aminobutyric acid in tea was extracted, isolated and determined. Water achieve a better effect than ethanol in extracting γ-aminobutyric acid, and γ-aminobutyric acid content was enhanced by 20%; 732 positive ion resin columns (2.5×50cm) was used to isolate, citric acid and ammonia water were used as elutes, based on pH linear gradient. Results indicated that γ-aminobutyric acid inelute could reach 39%; thin layer scanner could measure the content of γ-aminobutyric acid.更多还原