【作者】 侯渝嘉； 何桥； 李品武； 梁国鲁； 彭萍； 邓敏；

【Author】 HOU Yu-jia, HE Qiao, LI Pin-wu, LIANG Guo-lu, PENG Ping, DENG Min (Chongqing Tea Researcfh Institute, Yongchuan, Chongqing 402160. China;Southwest University, Chongqing 400716, China;Sichuan Agricultural University, Yaan, Sichuan 625014, China)

【机构】 重庆市茶叶研究所； 西南农业大学； 四川农业大学；

【摘要】 采用ISSR分子标记技术对川渝茶树资源进行了遗传多态性分析。结果表明川渝茶树资源遗传多态性丰富,12条ISSR引物共产生100条扩增带,其中多态性条带 90条(占90．0％)。ISSR标记遗传相似系数(GS)变异范围为0．41～0．87。通过类平均聚类(UPGMA)法,可将14份材料分为2个大类,第1大类主要为保持了较多原始性状的乔木大叶型,第2大类又可分成4个亚类群,主要为小乔木或灌木中小叶型,更多还原

【Abstract】 ISSR marker was used to analyze the genetic polymorphism of Sichuan-Chongqing tea germplasms. The results showed that the genetic polymorphism of these materials were abundant. 100 bands were amplified with 12 ISSR primers, and 90 bands of these were polymorphic (about 90%). The genetic similarity of the materials varied between 0.41 and 0.87. The 14 materials were classified into 2 groups with UPGMA method, the first group is original arbor and large-leaf type, the second one is semi-arbor or shrub type. And the second could be classified into 4 subgroups.更多还原