【作者】 王新超； 陈亮； 马春雷； 张亚丽； 梅菊芬； 赵丽萍； 姚明哲；

【Author】 Wang Xin-chao, Chen Liang, Ma Chun-lei, Zhang Ya-li, Mei Ju-fen, Zhao Li-ping, Yao Ming-zhe (Key Lab of Tea Chemical Engineering, Ministry of Agriculture,Tea Research Institute Chinese Academy of Agricultural Sciences, Hangzhou 310008, China)

【机构】 中国农业科学院茶叶研究所农业部茶叶化学工程重点实验室；

【摘要】 建立了用反相高效液相色谱测定茶叶中β-胡萝卜素含量的简便方法。样品先用丙酮浸提,真空旋转蒸干,再加乙醚和甲醇-氢氧化钾低温避光皂化3 h,然后用10％氯化钠萃取,乙醚相真空旋转蒸干。最后溶解于甲醇:乙酸乙酯混合液(1:1)。色谱方法如下:HypersiI C18ODS柱,流动相为丙酮和水,梯度洗脱,检测波长为450nm。分析结果表明,本方法重现性好,平均加样回收率为99．2％,RSD为4．5％。用该方法对龙井43不同季节、不同叶位的叶片和99份茶树资源的春季1芽2叶进行了β- 胡萝卜素的测定。结果发现,同一品种不同季节和不同叶位叶片的β-胡萝卜素含量差别很大。茶树叶片β-胡萝卜素含量是成熟叶高于幼嫩叶片,夏秋季高于春季。不同资源间β-胡萝卜素含量差别也很大。最高的为云桂大叶,含量达707．4mg／kg干重,最低的为黔湄601,仅为16．8 mg／kg干重,相差达42．1倍。更多还原

【Abstract】 A simple high performance liquid chromatographic analysis method of tea β -carotene has been developed. Firstly, samples were leached by acetone and reduced in the vacuum rotation evaporator. Secondly, samples were saponified by addition of diethyl ether and methanolic KOH, and allowed to rest for 3h at 4℃ with light excluded, and extracted by 10% NaCl, then the diethyl ether phases were reduced in the vacuum rotation evaporator. Finally, residues was solvated with methanol: ethyl acetate (v: v=1:1). The separation system consisted of a C18 reversed-phase column, a gradient elution system of acetone and water and detection wavelength of 450 am. While the content of β-carotene ranged between 2.5～100μg/mL, the method showed a good linear relationship between the peak area and content of β-carotene (r2=0.9936) and high reproducibility. The average rate of recovery of β-carotene was 99.2 % and the relative standard deviation (RSD) was 4.5 %. Beta-carotene contents of different positional, seasonal leaves of one tea plant variety Longjing 43 and additional 99 tea germplasms were measured using this method. It can be concluded that the content of β-carotene is higher in mature leaves than in immature leaves, summer and autumn shoots higher than spring shoots. The contents of β-carotene are of significant difference among germplasms. Yungui Daye, the highest germplasm, is 42 times higher than Qianmei 601, the lowest one.更多还原