【作者】 娄桂予； 钱民章；

【Author】 LOU Gui-Yu, and QIAN Min-Zhang( Department of Biochemistry, Zunyi Medical College , Zunyi 563003, China)

【机构】 遵义医学院生物化学教研室；

【摘要】 为观察橙皮甙和芦丁2种结构类似的黄酮类化合物对实验性家兔动脉粥样硬化的保护作用及对动脉壁单核细胞趋化蛋白1mRNA表达的影响,探讨防治动脉粥样硬化的新思路,采用高脂饮食加免疫损伤的方法复制家兔动脉粥样硬化模型,在建立模型的基础上喂饲橙皮甙(2g/天)或芦丁(0.4g/天)。10周后处死动物,检测血脂、血清丙二醛、一氧化氮水平及主动脉升部、弓部大体和镜下的病理变化,用硫氰酸胍法提取胸主动脉总RNA,逆转录—聚合酶链反应及图像分析检测单核细胞趋化蛋白1mRNA表达水平。结果表明,橙皮甙和芦丁减轻了高脂血症引起的家兔动脉粥样硬化的病变,抑制了动脉壁内单核细胞趋化蛋白1mRNA的表达。提示橙皮甙和芦丁减轻动脉粥样硬化病变的作用可能与其降血脂、抗氧化减少氧化型低密度脂蛋白的生成、升高血清一氧化氮含量,从而下调单核细胞趋化蛋白1基因表达有关。更多还原

【Abstract】 Aim To study the effects of hesperidin and rutin on prelecting from atherosclerosis and arterial monocyte chemoattractant protein-1(MCP-1) gene expression in the rabbits with atherosclerosis (As). Methods The model of atherosclerosis was established by feeding high lipid forage and immune injuring, then treated with hesperidin(2 g/d) and rutin(0.4 g/ d). After ten weeks we killed animal and examined the levels of their serum lipids, malondialdehyde(MDA), nitric oxide (NO) and the aortic pathology under naked eye and microscope. Total RNA of thoracis aorta was isolated by acid guanidinium thiocya-nate method. MCP-1 mKNA expression levels were determined by the reverse transcription polymerase chain reaction(RT-PCR) method. Results Hesperidin and rutin could mitigate the atherosclerosis which caused by hyperlipidemia and inhibite MCP-1 mRNA expression in thoracis aorta of rabbits. Conclusions The acts of hesperidin and rutin on antiatherosclerotic in rabbits might be associated with decreasing serum lipids and oxidized low density lipoprotein or increasing NO, which can down-regulated MCP-1 mRNA expression level.更多还原