【作者】 高洪； 唐兆新； 陈万芳； 彭辂； 赵汝； 杨建发；

【Author】 Gao Hong, Tang Zhaoxin, Chen Wanfang, Peng Lu, Zhao Ru, Yang Jianfa ( College of Animal Science and Techology, Yunnan Agricultural University ,Kunming 650201, China; College of Animal Mectcine,Nanjing Agricutural University,Nanjing 210095,China)

【机构】 云南农业大学动物科学技术学院； 南京农业大学动物医学院；

【摘要】 用电镜定性观察和体视学定量分析相结合的方法研究了大肠杆菌ET对山羊肝细胞超微结构损伤的体视学变化及654-2的保护作用.电镜定性观察发现,ET处理组(Ⅱ组)山羊肝细胞线粒体极度肿胀;胞浆内溶酶体数量增多,有的溶酶体外逸,可见大小不等、形状各异的髓鞘样小体;滑面内质网泡状增生,粗面内质网扩张、脱颗粒:高尔基器发达,枯否氏细胞增生;糖原颗粒数量增加、聚集;可见单个凋亡肝细胞,凋亡细胞内有小泡状结构:核仁数目增多,核仁成分分离,核仁和染色质边移.654-2和ET联合处理组(Ⅲ组)山羊也有类似变化,但程度较轻.体视学定量分析发现Ⅱ组山羊肝细胞线粒体平均截面积((?)_x)、平均截面积周长((?)_x》、平均外切直径((?)))、平均体积((?)))、体积密度(V_v)和面积密度(V_v)等除S_v与组差异不显著(P>0.05)外,均高于Ⅲ组和对照组(Ⅰ组)(P<0.01).数密度(N_v)、比面积(δx)和面数密度(N_A)等除N_A与Ⅰ组、δx与Ⅲ组差异不显著(P>0.05)外,均低于Ⅲ组和Ⅰ组(P<0.01,P<0.05);Ⅲ组山羊Vv、N_A和Sv显著高于对照组(P<0.01,P<0.05),其余参数均与Ⅰ组无显著差异.因而认为,ET会引起肝细胞及其膜性系统的广泛损害,线粒体损伤的主要特点为肿胀、数量减少和膜异形.654-2能有效防止这种损伤作用.更多还原

【Abstract】 Using techniques of quantitative stereologv with qualitative observation we have studied ul-trastructural changes and protection of anisodamine in E. coliendotoxin induced hepatocellular of goats. The results of electron microscopic morphomertry revealed the marked swelling of mitochondria with destruction of cristae, an increase in the number of lysosome, appearing myeloid body, dilation of the cis-ternsae and vesicles - like proliferation of smooth endoplasmic retictlum, dilation of rough endoplasmic reticulum with concomitant loss of membrane - associated ribosomes, hypeftrophy of golgi apparatus, proliferation of kuffer cells, an increase in the number of glycogen, appearing single apoptosis hepatocytes, an in - crease in the number and component detachment of nucleolus, marginal immigration of nucleolus in hepatic cells of endotoxin - treated goats ( group II ) . Similar changes in the hepatocytes of anisodamine and endotoxin unitedly treated goats ( group III ) existed but were slighter. The stereologic quantificationand analysis of the hepatocyte mitochondria showed that mean profile area(Ax), mean perimeter( Bx), mean callper diameter(D), mean volume(V), voltlme density(Vv) and surface density(Sx) in groupll were higher than those in group III as well as normal goats(group 1)(P < 0.01) ,except no significant difference In Sv as compared to group III ( P > 0.05 ); Numerical density (Nonspecific surface(δx) and numerical density on area( NA ) were than those of group III and group I (P <0.01,P<0.05), except on significant difference in NA as compared to group I and in δx as compared to groupIII(P>0.05).VV, NA and Sv in group III significant higher than those of group I (P<0.01,P<0.05); Other values didn’t show any significant differences(P> 0.05) between groupIII and group I .These results indicated that E.coli endotoxin could induce extensive damage of hepatocytes and their membrane systems;injury of mitochondria was characterized by severe swelling , reduced number and deformation, whereas aniso-damine could effectively protect against the endotoxin induced the ultrastrucmral damage in hepatocytes of goats.更多还原