【作者】 赵振华； 李向宇； 关平原； 日穆德玛； 赵心力； 顾玉芳； 吉仁泰； 吴钟灵； 成子强；

【Author】 Zhao Zhenhua Li Xiangyu Guan Pingyuan Ri Mudema Zhao Xinli Gu yufangJi Rentai Wu Zhongling Chang Ziqiang(Inner Mongolia Agriculture University Huhhot 010018,Inner Mongolia Husbandry Science Institute,Inner Mongolia Veterinary Station,Inner Mongolia Biology Medicine Factory)

【机构】 内蒙古农业大学； 内蒙古畜牧科学院； 内蒙古自治区兽医站； 内蒙古生物药品厂；

【摘要】 分别用AEV-NH937E_8.10.12制备的三个批号的禽脑脊髓炎油乳剂灭活疫苗按使用说明书给16～18周龄的成鸡接种免疫,通过定期检测血清AE琼扩抗体效价、攻毒以及所产种蛋鸡胚敏感试验、雏鸡血清检测和攻毒试验,对疫苗进行效力检验.结果表明,成鸡免疫后14天至12个月血清AE琼扩抗体效价≥1:4;免疫后6、12个月能抵抗强毒攻击而不发病——不出现AE症状、产蛋率不受影响、未发现脑组织有变质性病变;免疫1年内所产种蛋鸡胚敏感试验保护率94%以上,说明具有坚强的免疫力;孵出雏鸡在30日龄内血清AE琼扩抗体效价≥1:4,并能抵抗AE强毒攻击而得到保护.该疫苗效力确实符合我国《兽用生物制品质量标准》,为“禽脑脊髓炎油乳剂灭活疫苗的制造检验试行规程（草案）”的制定提供了效力检验方面的依据.成鸡免疫期定为一年.更多还原

【Abstract】 Chicken of 16 ~ 18 days age were immunized with three batches avian encephalomylitis inactivated oil emulsion vaccine prepared by AEV - NH937 E8,10,12 stains respectively according to instruction. The effect test of this vaccine was examined by agar diffusion, challenge, chicken embryo sensitive test from laying hen, serum examination and challenge test from young chicken. The results indicated that the agar diffusion liter were up 1:4 after the immunity 14 days to 12 months;The chicken can be against the strong AEV strains after the immunity 6 and 12 months,namely the chicken showed no AE symptom,egg drop and degenerative change in brain; The protective rate of chick embryo sensitive test from laying hen was 94 ~ 100% ;Progeny chicken serum AE agar diffusion liter was up1 :4, so they can be protected by this vaccine. This vaccine effect was reliable and it accord with National "Quality of Veterinary biology produce standard" and this paper provided the evidence of effect test to make""The prduce test try out regulation of avian encephalomylitis inactivated oil emulsion vaccine (draft)" . The immune period was lyear.更多还原