【作者】 陈创夫； 余兴龙； 乔军； 涂长春； 李作生； 张高轩； 田晶华；

【Author】 Chen chuangfu Yu xinglong Qao jun Li zhuosheng Tu changchun Yin zhen(Shihezi University, Shihezi Xinjiang 832003) (The Military Veterinary Institute Quartermaster University of PLA, Changchun 130063)

【机构】 石河子大学； 解放军军需大学；

【摘要】 应用FACS、MTT法对猪瘟基因疫苗pcDST、pcDSW免疫鼠外围血中CD₄⁺和CD₈⁺淋巴细胞数、淋巴细胞的转化功能等动态变化进行了观察.结果:pcDST、pcDSW猪瘟基因疫苗免疫小鼠后,小鼠的外周血淋巴细胞对LPS有强烈的反应性,CD₄⁺细胞数量增加,外周血淋巴细胞CD₈⁺细胞数量在一段时间内增加,CD₄⁺和CD₈⁺细胞数量在免疫后32天达到高峰后开始下降.表明pcDST、pcDSW质粒免疫小鼠后诱发免疫反应.更多还原

【Abstract】 FAGS, MTT were used for detecting the number of CD4+ and CD8+ T cells in splenocytes and peripheral blood lymphocytes of mice vaccinated with CSFV DNA vaccines, the mitogenic responsibilityto ConA and LPS, The results demonstrated that The splenocytes and peripheral blood lymphocytes, from mice vaccinated with CSFV DNA vaccines, responsed to ConA and LPS 20 days after vaccinating, the number of CD4+ and CD8+ T cells reached the peak on 32th day after vaccinating. . Summary: CSFV DNA vaccine can induce both humarol and cell mediated immunity;更多还原