【作者】 冯敏； 朱佳廷； 刘春泉；

【Author】 FENG Min ZHU Jia-ting LIU Chun-quan (Institute for Application of Atomic Energy in Agriculture, Jiangsu Academy of Agricultural Sciences Jiangsu nanjing 210014)

【机构】 江苏省农业农科院原子能所；

【摘要】 提供一种快速检测农产品含菌量的方法已成为辐射加工行业目前迫切需要解决的问题。本文应用ATP发光技术分别对辐照前、后农产品中的含菌量进行了研究。结果表明:①测定待辐照样品中的含菌量,从取样到获取结果,整个过程只需 1-2小时,而且样品的发光强度与含菌量呈显著正相关。②应用该方法检测农产品的辐照灭菌效果时,结果却出现了发光强度均高于对照的异常现象。深入研究发现, 发光强度的增加是由农产品中含有的细菌经辐照后引起的干扰;同时荧光素酶-荧光素制品中存在的能引起光辐射的物质是形成干扰的另一个方面的原因。更多还原

【Abstract】 The microbial colony-forming unit (cfu) in un-irradiated samples was determined by ATP bioluminescence method. The detecting time from preparation to determination is about 1-2h and value of ATP bioluminescence is correlative to the microbial cfu obviously. Using this technique as a rapid method to inspect irradiation sterilization effect, there was such an abnormal occurrence that ATP bioluminescence value of irradiated sample was higher than that of CK. In order to make clear the phenomenon, effect of γ -ray on ATP, dynamic analysis of luminescence and possible pathway of its intensity increase were investigated. The results showed that the increase of luminescence in irradiated food was not due to ATP, but mainly to the influence of the irradiated bacteria in sample, and probably to some other materials in luciferin-luciferase that may cause the abnormity.更多还原