【作者】 任慧霞； 张敏；

【Author】 REN Hui-xia ZHANG Min,Institue of Biochemical and Biotechnological Drug,School of Pharmaceutical Science,Shandong University,Jinan 250012

【机构】 山东大学药学院生化与生物技术药物研究所；

【摘要】 目的研究黄芩苷酶的制备及其理化性质。方法采用提取、透析、sephadex G150凝胶柱层析及冷冻干燥的方法获得黄芩苷酶;利用Linewear-Burk作图法求得该酶对黄芩苷的Km值,研究了pH值、温度、离子强度、底物浓度等对黄芩苷酶活力的影响。结果该酶对黄芩苷的Km值为57.4mmol/L,最适pH值为5.8,温度为45℃,不同离子的影响、分子量,底物浓度及产物对该酶活性基本无影响,反应初速度随酶浓度的增加而增加并呈现良好的线性关系。结论获得活性较高理化性质较稳定的一种黄芩苷酶。更多还原

【Abstract】 Purpose To measure the Km of this flavonoids Glycosidase from Scutellaria baicalensis Georgi with baicalin,study the effect of pH,temperature,ionic strength and subst rate concent ration on enzyme activity,and the linear relation of enzyme concentration.Methods Using means of collection and dislysis,sephadex G 150 column chromatography to prepare crude enzyme.Measuring Km by Linewear-Burk graphic method,calculate the enzyme activity by measuring the absorbance of baicalein at 450nm with ultraviolet spectrophotometry.Results The Km with baicalein is 57.4mmol/L,its optimum pH,temperature,ionic strength and subst rate concent ration is 5.8,45℃,1mol/L,40mmol/L,respectively.Conclusion The physico-chemical property of this new flavonoids Glycosidase from Scutellaria baicalensis Georgi was studied.更多还原