【作者】 顾慧芬； 庄意丽； 梅其春；

【Author】 GU Hui-fen1,ZHUANG Yi-1i1,MEI Qi-chun2(1.Shanghai Institute of Chinese Materia Medica,Shanghai 201203,China;2.School of Life Sciences,Fudan University,Shanghai 200433,China)

【机构】 上海市中药研究所； 复旦大学生命科学学院；

【摘要】 采用分子系统学方法分析铁皮石斛试管苗及其近缘种的遗传多样性,为准确进行基源鉴定、阐明属内的种间关系及发现新的药用资源提供分子证据。采集三十多种石斛样品分离提取DNA,PCR扩增ITS区及5.8S rDNA完整序列并测序,用Mega3.1软件进行系统学分析。采用邻接法和最大简约法分析铁皮石斛试管苗亲缘关系,对三十多种石斛类植物的ITS区序列进行分析,两种方法得到的系统树基本一致。ITS序列在石斛种间存在极其显著的差异,铁皮石斛与其它各种石斛均有各自特异性的序列位点,树状图显示铁皮石斛试管苗与野生铁皮石斛源于同一物种,而石斛属其它种与这两种遗传关系较远。更多还原

【Abstract】 Molecular systematic techniques were applied to reveal the genetic diversity of medicinal plant of Dendrobium candidum and their related species in Dendrobium.The internal transcribed spacer(ITS)as well as 5.8S rDNA sequences of more than 30 samples of Dendrobium were amplified using PCR method and sequenced.Mega 3.1 was used to analyze the genetic diversity within genus.Phylogenetic relationships among species in Dendrobium and tissue culture seedlings of Dendrobium candidum as outgroup were estimated by maximum parsimony and neighbor—joining methods with Mega 3.1 software,and similar ITS trees were obtained with the two methods.The results of phylogenetic analysis also indicate that the tissue culture seedlings of Dendrobium candidum has a closed relationship with Dendrobium candidum,while both Dendrobium candidum and tissue culture seedlings of Dendrobium candidum are the most distant to the other species of Dendrobium.更多还原