【作者】 林强； 王鑫； 葛喜珍； 桑帅； 高伟；

【Author】 Lin qiang1*,Wang xin1.2,Ge xizhen1,Sang shuai1,Gao wei1(1.Biochemical Engineering College of Beijing Union University,beijing 100023,China;2.College of Life Science and Technology,Beijing University of Chemical Technology,beijing 100029,China)

【机构】 北京联合大学生物化学工程学院； 北京化工大学生命科学与技术学院；

【摘要】 目的:探讨不同分子量壳寡糖对糖尿病大鼠的作用机制。方法:Wistar大鼠采用高脂高糖饮食同时腹腔注射链脲佐菌素复制糖尿病大鼠模型。将动物随机分为正常对照组、模型组、阳性对照和药物治疗组。给药两周后进行糖耐量试验。四周后处死大鼠取血清测定血糖、果糖胺和血脂各项指标。结果:与正常组比较,模型组血糖、果糖胺水平明显升高(P<0.01),血脂各项指标明显变化(P<0.01)。与模型组比较,治疗组各项指标有明显改善(P<0.05,P<0.01),且这种作用具有剂量和分子量依赖性。结论:本模型大鼠血糖升高,并引起脂代谢紊乱,分子量1700的壳寡糖、大鼠日用剂量为140mg.kg-1适合高脂糖尿病患者,可降低血糖、调节血脂,起到保健作用。更多还原

【Abstract】 Objective:To investigate the influence of different low molecular chitooligosaccharides in diabetic rats.Methods:Diabetic model was induced by injecting with streptozotocin(STZ)and the rats were treated with high fat and sugar diet for four weeks.Rats were randomly divided into four groups:normal group,model group,positive control group and different chitooligosaccharides treatment group.Rats were treated with chitooligosaccharides for two weeks,the glucose tolerance were tested.Blood sample was taken out from rats at the end of the experiments after 24 hours’ fasting for determination of the blood glucose,fructosamine and serum lipoprotein.Results:Comparing with the normal group,the blood glucose,fructosamine and serum lipoprotein were obviously different(P<0.01).The above mentioned index was improved(P<0.05～0.01)at the end of the experiments.And the effect was determined by dosage and molecular.Conclusions:The best molecular of chitooligosaccharides was 1700,and the dosage was 140mg.kg-1.d-1 in this diabetic model.更多还原