【作者】 焦广玲； 于广利； 高昊东； 赵峡； 王培培； 耿美玉；

【Author】 JIAO Guang-ling YU Guang-li GAO Hao-dong ZHAO Xia WANG Pei-pei GENG Mei-yu (School of medicine and pharmacy,Ocean University of China,Qingdao 266003)

【机构】 中国海洋大学医药学院；

【摘要】 目的制备β-1,3/1,6-葡聚糖硫酸酯并进行生物活性研究。方法以具有β-1,3/1,6-葡聚糖结构的水溶性灰树花多糖GF6F2为原料,以三氧化硫三甲胺盐（SO₃·Me₃N）为酯化试剂,合成了该多糖硫酸酯衍生物SGF6F2。结果所得产物酯化度为0.8,相对分子量为210 kDa。体外实验结果表明SGF6F2具有较好的抗凝活性及Src酪氨酸激酶抑制活性。结论灰树花多糖硫酸酯不仅具有一定的抗凝血活性,而且显示良好的抑制Src活性,为该糖类药物的开发提供了有用数据。更多还原

【Abstract】 Purpose To preparation ofβ-1,3/1,6-Glucans sulfate and study its bioactivities.Methods A water-soluble polysaccharide with structure ofβ-1,3/1,6-Glucans isolated from Grifola frondosa mycelium （GF6F2） was sulfated to SGF6F2 using sulfur trioxide trimethylamine complex （SO₃·Me₃N） as the reagent.Results Its degree of sulfation was found to be 0.8 and the relative molecular mass was 210 kDa.The bioassay in vitro results showed that SGF6F2 had a good anticoagulant activity and high Src inhibition ratio.Conclusion Theβ-1,3/1,6-Glucan sulfate not only showed good anticoagulation, but also inhibition to Src activity,these results offered useful data for polysaccharide based drug research..更多还原