【作者】 高建； 李俊； 麻兵继； 程文明； 金涌； 吕雄文； 闫晨； 王建青； 邵旭； 葛少祥；

【Author】 Gao Jian1, li Jun, Ma Bin-Ji2, Cheng Wen-Ming, Jin Yong, Lv Xiong-Wen, Yan Chen, Wang Jian-Qing, Shao Xu3, Ge Shao-Xiang3 ( Dept of Pharmacy, Anhui Medical University, Anhui Hefei 230032; 1. Dept of Pharmacy, The First Affiliated Hospital of Anhui Medical University, Anhui Hefei 230022; 2.KunMing Institute of Botany, Chinese Academy of Sciences, Yunnan Kunming 6502042;3.Hefei Seven Star Medicine and Technology Co. Ltd, Anhui Hefei 230088 )

【机构】 安徽医科大学第一附属医院药剂科； 安徽医科大学药学院； 中科院昆明植物所； 合肥七星医药科技有限责任公司；

【摘要】 目的优选玉屏风散中有效部位-玉屏风总苷(TGYPF)的制备工艺。方法设计L9(34)正交试验,采用HPLC-ELSD法测定黄芪甲苷的含量作为质控指标,考察乙醇浓度(A)、乙醇用量(B)、提取时间(C)和提取次数(D)四个因素对玉屏风总苷(TGYPF)提取的影响,优化提取工艺。结果最佳提取条件为:A2B3C3D2即80%乙醇,8倍量,提取3次,每次2h。结论该提取工艺稳定性和重现性良好,简便,快速,可行。更多还原

【Abstract】 Objective To study the optimized preparation process of total glucosides of Yu Ping Feng (TGYPF), one of the effective parts of Yu Ping Feng. Methods By use of orthogonal experimental design L9(34), four factors that may effect the extraction had been studied , including alcoholic concentration, alcoholic volume, extracting time and times. And the content of astragloside IV had been developed as its quality standard, screening out the best method of extraction conditions. Results The best extracting technical conditions were as follows: 8 times amount of 80%EtOH, extracting solvent to reflux for 2 hour and 3 times. Conclusion The optimized process is simple, fast and feasible, with its stability and repeatability are good respectively.更多还原