【作者】 陈伟光； 林霞； 睢凤英； 尹秀； 屠婕红； 盛静；

【Author】 CHEN Wei-guang1,LIN Xia1,SUI Feng-ying1, YIN Xiu2 ,TU Jie-hong1,SHENG Jing1 (Medical School of Jiaxing University, Jiaxing, Zhejiang 314001,China ;Medical College,Zhejiang University,Hangzhou 310006,China)

【机构】 嘉兴学院医学院； 浙江大学医学院；

【摘要】 目的研究葎草提取物抗结核菌活性及有效部位。方法以体外抑菌试验筛选葎草抗结核活性部位,采用试管倍比稀释法测定葎草提取物最低抑菌浓度(MIC),评价其体外抗菌活性。结果葎草的正丁醇萃取部位对结核分枝杆菌标准株H37RV及临床分离的敏感菌株具有较明显的抗菌作用,粗提物的MIC为125μg·mL-1。高浓度时1mg·mL-1有杀灭作用。结论葎草提取物对结核杆菌具有明显的抗菌活性,有进一步研究开发的价值。更多还原

【Abstract】 Objective To observe the activity of extracts of Humulus scandens against tuberculous bacillus and to find out the active fraction. Method The method of bacteriostasis in vitro was used to evaluate the Inhibitory Effects against Tuberculous Bacillus of the extracts from Humulus scandens. MIC of the extract were measured by the means of doubling dilution. Result the N-butanol extract had obvious antibacterial activity to H37RV and the sensitive strain which was separated from clinic. MIC of the extract was 125μg·mL-1 and the high levels (1mg·mL-1)of the extract had a killing activity. Conclusion Based on our experiments, extracts of H. scandens has obvious antimicrobial activity in vitro. It is valuable to be studied further.更多还原